Skip to main content
Addgene
Showing: 2601 - 2650 of 6249 results

  5. pAAV-hSyn-fDIO-EYFP-WPRE

    Plasmid
    #154870
    Purpose
    Human synapsin-1 promoter; Flp-dependent EYFP
    Depositor
    Ulrik Gether
    Insert
    EYFP
    Use
    AAV and Mouse Targeting; Flp-frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    154870-plasmid-map-sequence-id-308832

    Enlarge

  24. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

    Plasmid
    #35497
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35497-plasmid-map-sequence-id-196845

    Enlarge

  33. pAAV-TRE-FLEX-ChR2-YFP

    Plasmid
    #110337
    Purpose
    ChR2-YFP reporter vector of iTango and Cal-Light system for AAV production
    Depositor
    Hyungbae Kwon
    Insert
    tetO-Flex-ChR2-YFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Chr2 H134R
    Promoter
    tetO
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110337-plasmid-map-sequence-id-211135

    Enlarge

  36. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid
    #178057
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-FLAG
    Use
    AAV
    Tags
    FLAG-MBP
    Expression
    Mammalian
    Mutation
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178057-plasmid-map-sequence-id-349209

    Enlarge

  37. pAAV-Syn1-DIO-VGLUT2:miniSOG

    Plasmid
    #214200
    Purpose
    For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)
    Depositor
    Thomas Hnasko
    Article
    Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626
    Insert
    Slc17a6 tagged with miniSOG and hemagglutinin
    Use
    AAV and Cre/Lox
    Tags
    miniSOG (tagged with HA) is fused to the first in…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214200-plasmid-map-sequence-id-426873

    Enlarge

  41. pAAV EF1a DIO iChloC 2A dsRed

    Plasmid
    #70762
    Purpose
    An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E83Q,E90R,E101S,D156N,T159C
    Promoter
    EF1a
    Available since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    70762-plasmid-map-sequence-id-134333

    Enlarge

  43. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid
    #159920
    Purpose
    Mutagenesis of Vamp2
    Depositor
    Larry Zweifel
    Insert
    Vamp2 (Vamp2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159920-plasmid-map-sequence-id-317338

    Enlarge

  44. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Hiroyuki Hioki
    Article
    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    190236-plasmid-map-sequence-id-381571

    Enlarge

  45. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid
    #183765
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    ChR2-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183765-plasmid-map-sequence-id-361682

    Enlarge

  47. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

    Plasmid
    #114106
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002
    Insert
    Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114106-plasmid-map-sequence-id-252561

    Enlarge

  49. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid
    #27055
    Purpose
    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    GFAP
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27055-plasmid-map-sequence-id-12796

    Enlarge

  50. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50468-plasmid-map-sequence-id-75484

    Enlarge

Showing: 2601 - 2650 of 6249 results