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Showing: 1041 - 1060 of 11043 results

  2. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172316-plasmid-map-sequence-id-343650

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  3. MuHKII-pGFPN3

    Plasmid
    #21922
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKII cDNA sequence …
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only

  12. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid
    #172317
    Purpose
    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    172317-plasmid-map-sequence-id-338787

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  16. MuHKI-pGFPN3

    Plasmid
    #21919
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKI cDNA sequence w…
    Promoter
    Available since
    Nov. 2, 2009
    Availability
    Academic Institutions and Nonprofits only

  18. ATG-1929

    Plasmid
    #108712
    Purpose
    CBR2opt in pF4Ag expression vector with T7 and CMV promoter
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    T7, CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108712-plasmid-map-sequence-id-224541

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  20. CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

    Plasmid
    #117384
    Purpose
    An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Insert
    GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117384-plasmid-map-sequence-id-225349

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Showing: 1041 - 1060 of 11043 results