We narrowed to 1,221 results for: POLI

1. pCellFree_G03 GTF2H1

   Plasmid

   #67108

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2H1 (GTF2H1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_SBP-mCherry-CCR1

   Plasmid

   #222311

   Purpose

   Synchronize the trafficking of CCR1 from the ER.

   Depositor

   Franck Perez

   Article

   Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

   Insert

   Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Tags

   Cerulean

   Expression

   Bacterial

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRMC2-sec:msfGFP

   Plasmid

   #194914

   Purpose

   Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Expression

   Bacterial

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-mH33B-mClover3

   Plasmid

   #171496

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   H3.3B (H3f3b Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRMC2-tat:msfGFP

   Plasmid

   #194915

   Purpose

   Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Expression

   Bacterial

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMHE-mClover3-KASH5-DN

    Plasmid

    #171492

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMHE-GST-KASH5-DN

    Plasmid

    #171493

    Purpose

    T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    GST

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PVRL2-bio-His

    Plasmid

    #51824

    Purpose

    Expresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PVRL2 (NECTIN2 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid

    #89886

    Purpose

    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-NRasQ61K
    

    Use

    Unspecified

    Tags

    mCherry

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-KLF3

    Plasmid

    #49100

    Purpose

    Expresses KLF3 in mammalian cells

    Depositor

    Merlin Crossley

    Article

    Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

    Insert

    Krüppel-Like Factor 3(KLF3)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pD21-hgAPOAI

    Plasmid

    #124962

    Purpose

    Express ApoAI in the liver

    Depositor

    Kazuhiro Oka

    Article

    Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

    Insert

    human APOAI gene (LPA Human)
    

    Use

    Adenoviral

    Promoter

    human APOAI

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-KDEL_SBP-EGFP-CCR5

    Plasmid

    #222309

    Purpose

    Synchronize the trafficking of CCR5 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCRII apoBb.1

    Plasmid

    #121898

    Purpose

    Plasmid for making zebrafish apoBb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBb.1 (apobb.1 Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCRII apoA-IVb.1

    Plasmid

    #121903

    Purpose

    Plasmid for making zebrafish apoA-IVb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.1 (apoa4b.1 Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCRII apoA-IVb.2

    Plasmid

    #121904

    Purpose

    Plasmid for making zebrafish apoA-IVb.2 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.2 (apoa4b.2 Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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