We narrowed to 14,422 results for: nts

1. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Mutation

   601 has an additional mutation added 22 bp from d…

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 linker-RDF (GB2892)

   Plasmid

   #160604

   Purpose

   RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0.178

   Plasmid

   #119645

   Purpose

   Level 0 Part. Insertion site

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   7942 NS3 Down Flank
   

   Use

   Synthetic Biology

   Mutation

   4 nucleotide insertion between nt 65 and 66 in NS…

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0.165

   Plasmid

   #119634

   Purpose

   Level 0 Part. Insertion site

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   6803 NS1 Up Flank
   

   Use

   Synthetic Biology

   Mutation

   1 nucleotide insertion between nt 21 and 22 in NS…

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0.175

   Plasmid

   #119643

   Purpose

   Level 0 Part. Insertion site

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   7942 NS2 Up Flank
   

   Use

   Synthetic Biology

   Mutation

   bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVR2

   Plasmid

   #84719

   Purpose

   E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   12 nt stretch lacking A's inserted before 5&…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS2002

   Plasmid

   #239832

   Purpose

   Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Mutation

   Replacement of A12-A57 with GAAA in omegaRNA scaf…

   Promoter

   human U6

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-BLM

   Plasmid

   #80070

   Purpose

   Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

   Depositor

   Nathan Ellis

   Article

   Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

   Insert

   BLM (BLM Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   If correct ID, the cDNA is from HeLa and has char…

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ACPPiso1-V5

   Plasmid

   #113052

   Purpose

   Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

   Insert

   prostatic acid phosphatase isoform 1 (ACP3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACPPiso2-V5

    Plasmid

    #113053

    Purpose

    Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 2 (ACP3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

    Plasmid

    #221998

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-MEK1 S222TAG

    Plasmid

    #68302

    Purpose

    N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-MEK1 S218TAG

    Plasmid

    #68301

    Purpose

    N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S218TAG (MAP2K1 Synthetic, Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mutpRL-TK 3xUTR

    Plasmid

    #40758

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    3 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pPKm-195

    Plasmid

    #90496

    Purpose

    pcDNA3 - CMVmin - PhyB - MTAD, expresses PhyB and MTAD, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PhyB NT-MTAD
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-AtmiR173aTS-B/c

    Plasmid

    #227964

    Purpose

    Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-NbmiR482aTS-B/c

    Plasmid

    #227966

    Purpose

    Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. psicheck2-B4GALT3

    Plasmid

    #203601

    Purpose

    Reporter plasmid carrying B4GALT3 3'UTR

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    nt 1800-2330 3'UTR

    Promoter

    SV40

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ELL2mRNA+polyA

    Plasmid

    #127256

    Purpose

    For in vitro translation of human ELL2 with 30 nt pA tail

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    shortens 3'UTR to 40 bp adds A30 tail

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mutpRL-TK 6xUTR

    Plasmid

    #40761

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    6 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only