Skip to main content
Addgene
Showing: 1041 - 1060 of 1628 results

  17. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

    Enlarge

  18. pLSLT

    Plasmid
    #51498
    Purpose
    pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    T30 TALEN pair
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51498-plasmid-map-sequence-id-78599

    Enlarge

  19. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

    Enlarge

  20. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183027
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183027-plasmid-map-sequence-id-360504

    Enlarge

Showing: 1041 - 1060 of 1628 results