We narrowed to 21,630 results for: ESC;

1. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC1-SypHer3s-dmito

   Plasmid

   #108119

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-dmito
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-G-Flamp1-mut

   Plasmid

   #188568

   Purpose

   control probe of G-Flamp1, insensitive to cAMP

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1-mut
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1-SypHer3s-IMS

   Plasmid

   #108120

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-IMS
   

   Use

   Tags

   ratiometricYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-R-diLACCO1

   Plasmid

   #208028

   Purpose

   Control biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-diLACCO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-gRNA

   Plasmid

   #158973

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   gRNA3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ExRai-AktAR2

   Plasmid

   #184047

   Purpose

   Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   ExRai-AktAR2
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-HA-deLACCO1-NGR

   Plasmid

   #208024

   Purpose

   Control biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   deLACCO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Non-specific sgRNA

   Plasmid

   #109432

   Purpose

   MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Non-specific gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Apple-53BP1trunc

    Plasmid

    #69531

    Purpose

    Fluorescent reporter for DNA damage

    Depositor

    Ralph Weissleder

    Article

    Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Apple fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GPHN-FingR-mGold2s-CCR5TC

    Plasmid

    #231785

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold2s-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_NSlmb-vhhGFP4

    Plasmid

    #35579

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-BBS2

    Plasmid

    #218712

    Purpose

    Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS2 (BBS2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFEB promoter-luciferase reporter

    Plasmid

    #66801

    Purpose

    Reporter for activity of TFEB gene promoter

    Depositor

    Albert La Spada

    Article

    Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

    Insert

    TFEB promoter (Tfeb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-Raichu-RhoA-CR

    Plasmid

    #40258

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Raichu-RhoA-CR (RHOA Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPGK-SB13

    Plasmid

    #236078

    Purpose

    SB13 transposase, for use with Sleeping Beauty transponson plasmids

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Insert

    SB13 transposase
    

    Use

    Sleeping beauty (sb) transposase plasmid

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-TetON-3G

    Plasmid

    #96963

    Purpose

    Mammalian expression of Clontech rtTA driven by human beta-actin promoter

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. N1-Tq-Ca-FLITS

    Plasmid

    #191454

    Purpose

    Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    Tq-Ca-FLITS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid

    #100843

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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