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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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aP2-GFPtpz

Plasmid

#114211

Purpose

aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

Depositor

David Rowe

Article

Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

Insert

aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Aequorea victoria, Mouse, Synthetic)

Tags

None

Expression

Mammalian

Promoter

aP2/Fabp4

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTnr#2/Cre

Plasmid

#193245

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgTnr#2 (Tnr Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MED19

Plasmid

#15420

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pStreptagII 3C SMO

Plasmid

#234995

Purpose

Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

SMO (SMO Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nm-EBFP2

Plasmid

#231185

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_POU2F3_CR

Plasmid

#122175

Purpose

Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

3 silent mutations for gRNA resistance (882g-c, 8…

Promoter

EFS

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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nAChR beta2 WT

Plasmid

#24272

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR beta2 WT (Chrnb2 Mouse)

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-TIR1-HA

Plasmid

#172610

Purpose

Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

TIR1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-PcKRS

Plasmid

#140022

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

PcKRS (pylS Methanosarcina bakeri)

Tags

FLAG

Expression

Mammalian

Mutation

M241F, A267S, Y271C, L274M

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
pcw57.1-RquA-FLAG

Plasmid

#229685

Purpose

Doxycycline-inducible expression of RquA-FLAG

Depositor

Jessica Spinelli

Article

Valeros et al Cell. 2025 Jan 10:S0092-8674(24)01420-X.

Insert

RquA-FLAG

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MED20

Plasmid

#15421

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED20 (Med20 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Article

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EphrinB3-Flag

Plasmid

#155012

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-mLin28A

Plasmid

#26357

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog A (Lin28a Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Expression

Mammalian

Mutation

S472A

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3/BLM

Plasmid

#111766

Purpose

To express human BLM protein in human cells

Depositor

Ian Hickson

Article

Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

Insert

BLM (BLM Human)

Tags

Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Frt-hspB5

Plasmid

#63096

Purpose

Expression of HSPB5 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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