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pETM33_AP2B1_adaptin

Plasmid

#178467

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

AP2B1_adaptin (AP2B1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-HA-mouse GARP

Plasmid

#180258

Purpose

Mouse full length GARP expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

GARP (Lrrc32 Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET-NlaCascade-NLS-6xHis

Plasmid

#180213

Purpose

Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas5-Cas8-Cas7

Tags

6XHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPN10-gCTG

Plasmid

#114385

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCTG

Use

CRISPR

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a(+)- PC-MjMAT

Plasmid

#179372

Purpose

PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

Depositor

Andrea Rentmeister

Article

Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

Insert

PC-MjMAT

Tags

6xHis

Expression

Bacterial

Mutation

L147A, I351A

Promoter

T7

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Hras

Plasmid

#180573

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab5

Plasmid

#174455

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mito-roGFP

Plasmid

#174181

Purpose

Express roGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

roGFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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PRDX1A-c001

Plasmid

#175473

Purpose

Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

Depositor

Opher Gileadi

Insert

PRDX1 (PRDX1 Human)

Tags

AviTag and His6-TEV

Expression

Bacterial

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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2XSmBiT-SARS-CoV-2 S1

Plasmid

#173430

Purpose

protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)

Use

Luciferase

Tags

human IgG1 Fc

Expression

Mammalian

Mutation

furin cleavage site RRAR to GSAS

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EPB41L3A-c000

Plasmid

#175458

Purpose

Protein expression in bacterial cells. Entire CDS, M1-D1087.

Depositor

Opher Gileadi

Insert

EPB41L3 (EPB41L3 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)-mSandy2

Plasmid

#177760

Purpose

Expresses mSandy2 using the T7 Promoter

Depositor

Roberto Chica

Article

Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. d

Insert

mSandy2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAPV-DsRed2-ER-5

Plasmid

#170570

Purpose

Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

APV-DsRed2-ER-5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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AF1521-c002

Plasmid

#173109

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

mCerulean

Expression

Bacterial

Mutation

KH-M1-L192

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRiboI-APEX2m

Plasmid

#176843

Purpose

Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Use

Integrating

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parp14-c012

Plasmid

#173099

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

W1003-A1190

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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