159,352 results

1. TetO-FUW-NfiA

   Plasmid

   #64901

   Purpose

   Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

   Depositor

   Vania Broccoli

   Article

   Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

   Insert

   Nfia (Nfia Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Piggybac-TetO-hNurr1-blast

   Plasmid

   #176485

   Purpose

   TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Nurr1 (NR4A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTEF:ATP

   Plasmid

   #92179

   Purpose

   HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
   

   Use

   Integration and expression into yeast.

   Tags

   Yeast codon optimized mseCFP (donor) and cp173-mV…

   Expression

   Yeast

   Mutation

   Promoter

   TEF1p

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(-)-flag-UBR2

   Plasmid

   #50573

   Purpose

   Expresses FLAG-tagged mouse UBR2 in mammalian cells.

   Depositor

   Alexander Varshavsky

   Insert

   UBR2 (Ubr2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAVS1-P-CAG-GFP

   Plasmid

   #80491

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   CAG-GFP
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDH-puro-CMV-RC3AI

   Plasmid

   #78908

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   RC3AI
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

9. LeGO-C2

   Plasmid

   #27339

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSC-humanADAR1

    Plasmid

    #198235

    Purpose

    Expresses human ADAR1 in yeast

    Depositor

    Brenda Bass

    Article

    Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

    Insert

    human ADAR1 (ADAR Human)
    

    Use

    Tags

    His10_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSP3535

    Plasmid

    #46886

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Sst44-GFP

    Plasmid

    #172310

    Purpose

    SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst44
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pB-Globin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-FLEX-P301L Tau

    Plasmid

    #176267

    Purpose

    This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

    Depositor

    Tsuneya Ikezu

    Article

    You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

    Insert

    Flex-P301L tau (MAPT )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-ACE2-GFP

    Plasmid

    #154962

    Purpose

    pcDNA3.1-ACE2-GFP

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    ACE2-GFP (ACE2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge