164,826 results

1. pMTMVi-M

   Plasmid

   #118757

   Purpose

   Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus cDNA fragment with a cloning polylinker
   

   Use

   Intermediate to build tobacco mosaic virus recomb…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. STING-V2

   Plasmid

   #124264

   Purpose

   C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Use

   Tags

   Venus2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_MYC_WT

   Plasmid

   #82927

   Purpose

   Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   Myc (MYC Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-MCU-V5-HIS

   Plasmid

   #31731

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Tags

   V5-HIS6X

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. hCas9_D10A

   Plasmid

   #41816

   Purpose

   Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9_D10A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A nickase

   Promoter

   CMV

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSerOTS-C1* (V70)

   Plasmid

   #188537

   Purpose

   Phosphoserine incorporation into recombinant proteins

   Depositor

   Jesse Rinehart

   Article

   Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

   Inserts

   pSerRS
   Ef-pSer
   tRNApSer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations resulting from laboratory evolution

   Promoter

   glnS*

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

   Plasmid

   #111727

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q54 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-GEM-GECO1

   Plasmid

   #32442

   Purpose

   Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYLCRISPR/Cas9Pubi-H

    Plasmid

    #66187

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-His-SCov2-nsp7

    Plasmid

    #154757

    Purpose

    Expression of SARS-CoV-2 nsp7

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRGEN-CMV-CjCas9

    Plasmid

    #89752

    Purpose

    Expression of CjCas9 in mammalian cells

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. OSCA3.1

    Plasmid

    #136596

    Purpose

    Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA3.1 (ERD4 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mc2 puro

    Plasmid

    #206215

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1382

    Plasmid

    #111436

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-EGFR-T790M

    Plasmid

    #116310

    Purpose

    Lentiviral expression of EGFR T790M

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T790M

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 HA-Nprl2

    Plasmid

    #99709

    Purpose

    Mammalian expression of HA-Nprl2

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    Nprl2 (NPRL2 )
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-CMV-Galpha-oB ONE-GO

    Plasmid

    #189733

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oB ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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