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Mammalian_SpCas9_Target-AID3S

Plasmid

#188649

Purpose

Expresses SpCas9_Target-AID3S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID3S-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-TNFa-RUSH

Plasmid

#166902

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG2abc

Plasmid

#80617

Purpose

pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBa.Lamp1-GFP

Plasmid

#180250

Purpose

Mammalian expression of LAMP1

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG2abcde

Plasmid

#80619

Purpose

pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-ORP1

Plasmid

#191758

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBIG2abcd

Plasmid

#80618

Purpose

pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only
pMedium-FT-N1

Plasmid

#31911

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pBIG1c

Plasmid

#80613

Purpose

pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pB80-2xFKBP-L-mCherry-CAAX

Plasmid

#193713

Purpose

Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

2xFKBP-L-mCherry-CAAX

Tags

2xFKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAR357_SFP_pCDF-1b

Plasmid

#122851

Purpose

Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

Sfp

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rim2

Plasmid

#170869

Purpose

Mammalian expression of His-GFP-Rim2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

mCherry- eMagBF - 5ptaseOCRL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmPB3-VSV

Plasmid

#68039

Purpose

Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Tags

VSV

Expression

Mammalian

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-PMr

Plasmid

#61180

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRA

Plasmid

#183313

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRA

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps_LacZα_gIII

Plasmid

#190435

Purpose

M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

M13 packaging signal
M13 gIII
LacZα

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg30-PDGFR_EF1a_mCherry

Plasmid

#186309

Purpose

Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg30-PDGFR
mCherry

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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