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171,035 results

Showing: 10741 - 10760 of 171035 results

  1. pcDNA5-FRT-TOgw-C-mEGFP

    Plasmid
    #223953
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223953-plasmid-map-sequence-id-450835

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  2. pcDNA5-FRT-TOgw-C-mECFP

    Plasmid
    #223950
    Purpose
    Gateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mECFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223950-plasmid-map-sequence-id-450833

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  3. pcDNA5-FRT-TOgw-C-mEYFP

    Plasmid
    #223951
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEYFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223951-plasmid-map-sequence-id-450832

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  4. pcDNA5-FRT-TOgw-N-mEGFP

    Plasmid
    #223949
    Purpose
    Gateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223949-plasmid-map-sequence-id-450830

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  9. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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  10. TFORF3546

    Plasmid
    #145022
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TSC22D3 (TSC22D3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145022-plasmid-map-sequence-id-287180

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  11. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid
    #171810
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171810-plasmid-map-sequence-id-337982

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  17. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid
    #217319
    Purpose
    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.
    Depositor
    Noboru Mizushima
    Article
    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:
    Insert
    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    Use
    Retroviral
    Tags
    HaloTag and SNAP
    Promoter
    CMV
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217319-plasmid-map-sequence-id-436845

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  19. MultiMate-Rainbow

    Plasmid
    #206252
    Purpose
    Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
    Use
    Recombinant baculovirus production (bac-to-bac)
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206252-plasmid-map-sequence-id-406651

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Showing: 10741 - 10760 of 171035 results