We narrowed to 13,966 results for: GAN

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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PTRF/Cavin1_R (OZ514)

Plasmid

#27189

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-AREG-ScNeo

Plasmid

#209897

Purpose

To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

AREG-ScNeo (AREG Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

Plasmid

#208385

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgcGAS (Cgas Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

Plasmid

#115788

Purpose

Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Tags

3xflag

Expression

Mammalian

Mutation

contains silent mutation that creates an internal…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

Plasmid

#158654

Purpose

making transgenics expressing a QUAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-Y195F

Plasmid

#203573

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Tyrosine 195 to Phenylalanine for partial…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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CA-RIT-NFAT1 (IRES-GFP)

Plasmid

#85181

Purpose

Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

Depositor

Anjana Rao

Article

Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

Insert

CA-RIT-NFAT1 (Nfatc2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCDH-puro/Flag PD-L1 N35/3NQ

Plasmid

#121455

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N192Q, N200Q and N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 N192Q/N200Q

Plasmid

#121453

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N192Q/N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 N200Q/N219Q

Plasmid

#121454

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N200Q/N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUB6_EZH2

Plasmid

#84014

Purpose

EZH2 minigene to test splicing event

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

EZH2 (EZH2 Human)

Expression

Mammalian

Promoter

UB6

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-AREG-ScNeo

Plasmid

#209910

Purpose

To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

AREG-ScNeo (AREG Human)

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124-His

Plasmid

#246685

Purpose

Expression vector for secreted human GPR124 ECD, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Ad:2CMV_A2R2-N25K3

Plasmid

#122242

Purpose

Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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SOX9-T2A-H2B-EGFP repair template

Plasmid

#167972

Purpose

SOX9 reporter repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 9 (SOX9 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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