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We narrowed to 25,334 results for: Spr

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Showing: 10761 - 10780 of 25334 results

  5. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  7. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196600-plasmid-map-sequence-id-386793

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  8. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  11. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  15. qTAG-C-mScarlet-Puro-H2BC11

    Plasmid
    #207761
    Purpose
    Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207761-plasmid-map-sequence-id-415325

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  17. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid
    #207762
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207762-plasmid-map-sequence-id-416207

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  18. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

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Showing: 10761 - 10780 of 25334 results