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Showing: 2121 - 2160 of 8645 results

  6. pBB328

    Plasmid
    #221533
    Purpose
    Plasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.
    Depositor
    Brett Barney
    Inserts
    vioABCE
    Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
    Tetracycline antibiotic cassette from pRK415
    Expression
    Bacterial
    Promoter
    None, contains RBS site
    Available Since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221533-plasmid-map-sequence-id-442457

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  7. pBTK1061

    Plasmid
    #209550
    Purpose
    BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
    Use
    Synthetic Biology
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    209550-plasmid-map-sequence-id-418282

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  19. pSEVA12TEC-B

    Plasmid
    #58307
    Purpose
    GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    Genomic cassette
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58307-plasmid-map-sequence-id-89819

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  23. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  25. pADH110

    Plasmid
    #90982
    Purpose
    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    NAT 2 of 2
    pSNR52
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90982-plasmid-map-sequence-id-180510

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  26. pDT305

    Plasmid
    #128801
    Purpose
    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Depositor
    Bradley Bernstein
    Article
    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:
    Insert
    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Use
    CRISPR and Lentiviral
    Promoter
    hUBC promoter
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    128801-plasmid-map-sequence-id-255180

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  38. pSelAct-KO

    Plasmid
    #174374
    Purpose
    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Vector for 2-step knockout by homologous recombin…
    Promoter
    N/A
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174374-plasmid-map-sequence-id-356074

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Showing: 2121 - 2160 of 8645 results