We narrowed to 26,551 results for: CAT

1. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a-DAAT-V33G/T242G

   Plasmid

   #159278

   Purpose

   Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

   Depositor

   Roberto Chica

   Article

   Parmeggiani et al ACS Catalysis 9, 3482–3486.

   Insert

   Engineered d-alanine aminotransferase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Valine 33 and threonine 242 have been mutated to …

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEsg1-6K-Luc

   Plasmid

   #15394

   Depositor

   Shinya Yamanaka

   Article

   Amano et al BMC Dev Biol. 2006 Feb 28;6:11. doi: 10.

   Insert

   Upstream region of mouse Esg1 gene (Dppa5a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pET16b-RPA4/His14 (MSW#949)

   Plasmid

   #208075

   Purpose

   Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

   Depositor

   Marc Wold

   Article

   Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

   Insert

   RPA4, RPA14(His-tagged)
   

   Use

   Tags

   10xHis tag on RPA14

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE80l BCB

   Plasmid

   #196472

   Purpose

   Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   BCB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET3a-FruK

   Plasmid

   #186256

   Purpose

   Overexpression of FruK for purification

   Depositor

   Emile Van Schaftingen, Liskin Swint-Kruse

   Article

   Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

   Insert

   1-phosphofructokinase (fruK E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCHC042 (∆phaCAB)

   Plasmid

   #226208

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKPI-MtLAP1

   Plasmid

   #193521

   Purpose

   Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

   Depositor

   Jeremy Murray

   Article

   Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

   Inserts

   MtLAP1
   DsRed
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ATUBI10 and Medtr8g059790(KPI)

   Available since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTXB1.Tat(48-57)-Cre recombinase

   Plasmid

   #165042

   Purpose

   Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Mxe Intein-Chitin binding domain and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCHC005 (∆RE)

    Plasmid

    #226199

    Purpose

    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCHC039 (∆phcA)

    Plasmid

    #226207

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pL1-pKPI-MtLAP1-Adh

    Plasmid

    #193520

    Purpose

    Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

    Depositor

    Jeremy Murray

    Article

    Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

    Insert

    MtLAP1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Medtr8g059790(KPI)

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE80l B-CN-B

    Plasmid

    #196477

    Purpose

    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    B-CN-B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-GP-2-MPHOSPH8 Chromo

    Plasmid

    #59694

    Purpose

    Contains histone modification interacting domain (MPHOSPH8 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    MPHOSPH8 Chromo (MPHOSPH8 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172669

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    RNF2 pegRNA and RNF2_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1‐Afu‐rnhB D101N

    Plasmid

    #108695

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D101N catalytic site mutation

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MS2-adRNA (RAB7A-GAC site)

    Plasmid

    #170140

    Purpose

    AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-RAB7A(GAC)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2-adRNA (5'GAC)

    Plasmid

    #170127

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'GAC)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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