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pACEBac1-PSMA5-PSMA6-PSMA7

Plasmid

#217894

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB5-PSMB6-PSMB7

Plasmid

#217898

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

Plasmid

#181956

Purpose

Genetically encoded FRET-based cAMP indicator fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)

Use

Tags

AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-EGFP-TM-IRES-Pac

Plasmid

#183603

Purpose

Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKJ1267 (aka pAC-alphaGal4)

Plasmid

#21869

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Use

Tags

Expression

Yeast

Mutation

Promoter

Actine

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAC8_MF-p10-mCh (VE5626)

Plasmid

#139772

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

mCherry fluorescent protein

Use

Tags

Expression

Mutation

Promoter

PH

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

Plasmid

#216384

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

5BoxB

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Hyg-CTCF-ZnF-N1

Plasmid

#131802

Purpose

Expresses the [Zinc finger domain of human CTCF (a.a. 265-603)]-GFP in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-1731

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pActin5c-CD-dCas9m4-UDI-attB

Plasmid

#104879

Purpose

To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

Depositor

Christopher Potter

Insert

CD-dCas9(m4)-UDI

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

Plasmid

#79259

Purpose

Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

Depositor

Elisa Izaurralde

Article

Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

Insert

DmeIF4G (eIF4G1 Fly)

Use

Schneider 2 cell expression

Tags

V5-His6 and lambdaN-HA

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-pac-T2A-dre

Plasmid

#124833

Purpose

Puromycin resistance gene and dre recombinase

Depositor

Mario Capecchi

Insert

pac-T2A-dre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pC002 - LshC2C2 locus into pACYC184

Plasmid

#79151

Purpose

Endogenous LshC2c2 locus cloned into pACYC184

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAC95-pmax-dCas9VP160-2A-neo

Plasmid

#48227

Purpose

dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo

Use

CRISPR

Tags

2A-neo, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Hyg-CTCF-Nterm-N1

Plasmid

#131800

Purpose

Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 796-2181 (the N-terminus is l…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Hyg-CTCF-Cterm-N1

Plasmid

#131801

Purpose

Expresses the [C-terminus of human CTCF (a.a. 577-728)]-GFP in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-1731

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPBbsr-6080NES(Epac-sensor)

Plasmid

#162487

Purpose

hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Epac-sensor (RAPGEF3 Synthetic, Human)

Use

Tags

YPet and Turquoise2GL

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAceR-Kv2.1PR

Plasmid

#195531

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA scaffold-spacer-human U6

Plasmid

#214697

Purpose

provide "sgRNA scaffold-spacer-human U6" fragment

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

sgRNA scaffold-spacer-human U6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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