We narrowed to 2,983 results for: erf
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Plasmid#145177PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 6DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIBN-EYFP-hTRF1
Plasmid#103802PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorPublicationInsertLuxI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorPublicationInsertLasI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.23-MYC-e5
Plasmid#120343PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertchr8:130594100-130596043 (hg19)
UseLuciferaseExpressionMammalianPromoterMYCAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e4
Plasmid#120342PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertchr8:129060477-129062593 (hg19)
UseLuciferaseExpressionMammalianPromoterMYCAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 YY1
Plasmid#67082PurposeStandard for cell-free expression benchmarking.DepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJK2020
Plasmid#171932Purposeexpress MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operonDepositorInsertPmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
Tagssuperfolder GFP, mCherryExpressionWormPromoter-Available sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spa)
Plasmid#133318Purposeexpresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdcas9 (Streptococcus pasteurianus)
UseUnspecifiedPromoterxyloseAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ mWnt5a
Plasmid#102860PurposeExpresses mouse Wnt5a in mammalian cells, zebrafish or XenopusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMouse Wnt5a
UseZebrafish expression; xenopus expressionExpressionMammalianPromoterCMV; SP6Available sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCellFree_G03 GATA1
Plasmid#67068PurposeStandard for cell-free expression benchmarking.DepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralMutationdelta tat delta TAR delta env GFP in Nef Tet ope…Available sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct5C-Hh
Plasmid#37382DepositorAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-Puro-CTRLg1 (CG509)
Plasmid#139455PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#108770PurposeExpresses aa397 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta Br
Plasmid#13255DepositorInsertRepressor Activator Protein 1 delta Br (TERF2IP Human)
UseRetroviralTagsFLAGMutationcontains hRap1 aa2-44 + 96-399 and the point muta…Available sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only