We narrowed to 2,254 results for: levi

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pJH106

Plasmid

#243727

Purpose

Expresses a chimeric form of PMPCA, where the region between residue 100 and 351 is replaced by a 250-residue XTEN linker

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

PMPCA(1-100aa)-XTEN250-PMPCA(351aa-stop) (PMPCA Human)

Use

Lentiviral

Mutation

The region between residue 100 and 351 of PMPCA i…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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AAV Syn-FLEX-coca-GlyR-IRES-mCherry

Plasmid

#242196

Purpose

Cre-dependent AAV vector for cocaine-gated chemogenetic anion channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-FLEX-coca-GlyR-IRES-mCherry

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 3xFLAG COP1

Plasmid

#237334

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

COP1 (COP1 Human)

Expression

Bacterial

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B C186S

Plasmid

#230995

Purpose

Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Cys186 to Ser

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B L597A

Plasmid

#230994

Purpose

Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Leu597 to Ala

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET3a.H2B-K120C

Plasmid

#224706

Purpose

Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

H2B-K120C (H2B Human)

Expression

Bacterial

Mutation

K120C

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 R2896C

Plasmid

#139511

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 E2896C using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-IRES-hrGFP-mWnt3a

Plasmid

#89771

Purpose

Expresses mouse Wnt3a in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt3a (Wnt3a Mouse)

Use

AAV

Tags

None

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2

Plasmid

#220243

Purpose

Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pT-Rex Dest30 dn-cHSF1

Plasmid

#134736

Purpose

Mammalian expression vector for inducible expression of a constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV/TO

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pIDTSmart-CMV-PLRS1

Plasmid

#217365

Purpose

Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

Depositor

Abhishek Chatterjee

Article

Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

Insert

leucyl-tRNA synthetase polyspecific mutant

Expression

Mammalian

Mutation

M40I, T252A, Y499I, Y527A, H529G

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES_Tomato

Plasmid

#107229

Purpose

Tomato expressing plasmid

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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