We narrowed to 126,154 results for: CIL

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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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DRD4-Tango

Plasmid

#66271

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

DRD4 (DRD4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
ADORA1-Tango

Plasmid

#66209

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADORA1 (ADORA1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-mir-17-92

Plasmid

#64100

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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PGL3-NOXA-N1

Plasmid

#26112

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

NOXA N1 (PMAIP1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pTRACER LA

Plasmid

#15003

Depositor

Raymond Birge

Insert

LA (MFGE8 Human)

Tags

6xHis and V5

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-Fbx15

Plasmid

#13353

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

F-box only protein 15 (Fbxo15 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pICH47742:: pRPS5a-PE2max-NC::tHSP

Plasmid

#227694

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by pRPS5a and terminated by tHSP

Use

CRISPR

Expression

Plant

Promoter

pRPS5a

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMThy1.1_hDDX5_FL

Plasmid

#88873

Purpose

constitutive expression of DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTK229

Plasmid

#71256

Purpose

luciferase reposter with 229 bp of the HSV TK promoter

Depositor

Peter Cockerill

Article

Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

Insert

Thymidine Kinase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

HSV Thymidine Kinase

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overexpression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pγsat

Plasmid

#39238

Depositor

Niall Dillon

Article

Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Insert

8 copies of the 234bp mouse γ-satellite repeat

Use

Cloning vector

Available Since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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Map3k1

Plasmid

#29581

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Map3k1 (MAP3K1 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively Active. Contains catalytic domain …

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only