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pCMV-BE4-PpAPOBEC1 H122A

Plasmid

#138338

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1H122A

Depositor

Nicole Gaudelli

Publication

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1 H122A

Use

Synthetic Biology

Tags

BP-NLS

Mutation

Change of His122 to Ala.

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-SEC62

Plasmid

#139865

Purpose

Lentiviral expression of HA-tagged human SEC62

Depositor

Jacob Corn

Publication

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

SEC62 (SEC62 Human)

Use

Lentiviral

Tags

HA

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-CRY2-uTEV1Δ(220-242)

Plasmid

#135452

Purpose

Yeast expression of uTEV1Δ fused to CRY2 and mCherry

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-CRY2-TEV1Δ(220-242)

Tags

mCherry

Expression

Yeast

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC27A2

Plasmid

#131867

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A2 (SLC27A2 Human)

Expression

Mammalian

Available since

Oct. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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EF1a_KLF6_P2A_Hygro_Barcode

Plasmid

#120492

Purpose

Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF6 (KLF6 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

Plasmid

#104116

Purpose

synOptopatch construct

Depositor

Adam Cohen

Publication

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Publication

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PLK1-cjun-FRET-PACT biosensor

Plasmid

#106907

Purpose

centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

Depositor

Heidi Hehnly

Publication

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)

Tags

PACT domain

Expression

Mammalian

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon2-KGC-EGFP-ER2

Plasmid

#108426

Purpose

Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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IL10RB_pCSdest

Plasmid

#53799

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IL10RB (IL10RB Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFETCh_AHR

Plasmid

#101071

Purpose

Donor vector for 3' FLAG tag of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

AHR homology arms (AHR Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLostfoX-Kan

Plasmid

#99865

Purpose

TfoX induction vector for chitin-pathway transformation (KanR)

Depositor

Mark Mandel

Publication

Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

Insert

tfoXVF

Use

V. fischeri suicide cloning vector, cmr, tcr

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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BPTF PHD and Bromodomain

Plasmid

#92101

Purpose

Expresses GST fusion PHD and Bromodomain from BPTF protein

Depositor

Albert Jeltsch

Publication

Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

Insert

PHD and Bromodomain (BPTF Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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C-BLa 1.2

Plasmid

#89000

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Publication

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

C-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-MYRIP

Plasmid

#89586

Purpose

Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Publication

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYRIP (Myrip Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-Hsp27-S82A

Plasmid

#84993

Purpose

Hsp27 expression plasmid

Depositor

Ugo Moens

Insert

Hsp27 (HSPB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Serine 82 mutated to Alanine

Promoter

CMV

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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