We narrowed to 23,118 results for: Sis

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pcDNA5/FRT/TO HSPA2

Plasmid

#19458

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA2 (HSPA2 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+PIF6CreN

Plasmid

#131780

Purpose

Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PIF6CreN

Use

Cre/Lox; Zebrafish expression

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-T2A-mTagBFP2

Plasmid

#160500

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-RIPK3

Plasmid

#78827

Purpose

To purify GST-tagged recombinant RIPK3 protein

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLL3.7-K122-TEAD-p-H2B-mCherry

Plasmid

#139648

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

IRES-GFP

Use

Lentiviral

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAGFP

Plasmid

#58255

Purpose

U6 promoter-driven single guide RNA complementary to eGFP

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAGFP cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCK081 desmaMCS:minpromCerulean,exorh:mCherry

Plasmid

#200014

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302 5aa SunTag VP64 nog

Plasmid

#115480

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pRW004-SM-destination-proUBQ10-Cas9

Plasmid

#104438

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

UBQ10

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR-tagBFP-PSM-dTOMATO

Plasmid

#100604

Purpose

Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Type

Empty backbone

Use

Synthetic Biology

Tags

dTOMATO-T2A-Puro and tagBFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

Plasmid

#47387

Purpose

allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

glutamate-gated chloride Channels

Expression

Mammalian

Mutation

Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7·X-nia

Plasmid

#122592

Purpose

Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS/Pm->Nia

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mTagBFP2-MRTF-B

Plasmid

#182062

Purpose

Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-B (Mrtfb Mouse)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV300

Plasmid

#204968

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52545

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCGN-ELK-1 S383A

Plasmid

#27160

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

TAD mutant: S383A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pBAD24-rnc-sfGFP

Plasmid

#51560

Purpose

Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

Depositor

Sankar Adhya, Francisco Malagon

Article

Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

Insert

rnc (rnc E. coli)

Tags

GLESTCRHASLAVLADERRFSA and superfolder GFP

Expression

Bacterial

Mutation

Contains additonal aa at the end of the sfGFP in …

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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