We narrowed to 20,122 results for: Kin

1. pJL1-mRFP1 gRNA

   Plasmid

   #117052

   Purpose

   In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   anti-mRFP1 SpCas9 gRNA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGST TCF4 DBD

   Plasmid

   #16488

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   TCF4 DNA binding domain (TCF7L2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 265-496

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pFUW-TetO-SUB1

   Plasmid

   #178440

   Purpose

   doxycycline-inducible expression of Human SUB1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   SUB1 (SUB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSC1-D4Z4-GiP

   Plasmid

   #58542

   Purpose

   Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Human EF1-alpha

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-β-arrestin2 RRK/Q

   Plasmid

   #38261

   Depositor

   Robert Lefkowitz

   Article

   Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

   Insert

   β-arrestin2 (Arrb2 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Arg233, Arg237, and Lys251 converted to Glutamines

   Promoter

   CMV

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. MAC_N_ELK3

   Plasmid

   #167750

   Purpose

   Expresses ELK3 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   ELK3 (ELK3 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-CCHD (p130Cas)

   Plasmid

   #145133

   Purpose

   Expresses CCH domain from p130Cas with an mCherry tag

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   p130Cas CCH domain (BCAR1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-RapR-FAK-YM-Y397F

   Plasmid

   #25930

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-FAK (Ptk2 Human, Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-PS-Sos1 (PRM) - Cys (XSB364)

    Plasmid

    #78506

    Purpose

    Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Sos1 -Cys (PRM) (SOS1 Human)
    

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-gp120(BaL)-CD4

    Plasmid

    #98957

    Purpose

    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    HIV-1 (BaL) gp120
    

    Tags

    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

13. LENTICRISPR-PGAM1_sgRNA2

    Plasmid

    #201615

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. TRE-eGFP-F1C

    Plasmid

    #211457

    Purpose

    Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation blocking peptide (F1C)

    Depositor

    Linda DeGraffenried

    Article

    McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

    Insert

    Flotillin-1-palmitoylation blocking peptide (FLOT1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGW1-Myc-DJ1-L166P/K130R

    Plasmid

    #29349

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Leu166Pro and Lys130Arg

    Available Since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. TTR C10S S85C K80D

    Plasmid

    #184743

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80D

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKO.1 puro-luc (shRNA)

    Plasmid

    #153962

    Purpose

    Expresses a shRNA against firefly luciferase

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    shRNA against firefly luciferase (19-bp-long target)
    

    Use

    Luciferase, RNAi, and Retroviral

    Tags

    N/A

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IPU-FLAG-NEK9T210A

    Plasmid

    #168270

    Purpose

    Expresses kinase-dead NEK9 mutant in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 210 to alanine

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/GS-DJ1-L166P-V5

    Plasmid

    #29343

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    L166P

    Available Since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only