12,973 results

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pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

Plasmid

#184636

Purpose

Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Mutation

Gat1 gene with Arginine 69 changed to Lysine, to …

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for Hist1h3g

Plasmid

#186932

Purpose

Genomic targeting of SNAP tag at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for Hist1h3g (H3c8 Mouse)

Tags

SNAP

Expression

Mammalian

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Flag-Mcm2 targeting sgRNA

Plasmid

#186937

Purpose

Genomic targeting of Flag tag at Mcm2 N-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Daxx KO sgRNA

Plasmid

#186939

Purpose

Daxx KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Daxx KO sgRNA (Daxx Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP670-KASH2ext

Plasmid

#187022

Purpose

Control for inducible disruption of LINC Complex

Depositor

Jan Lammerding

Insert

KASH2ext

Tags

iRFP670

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Mcm2-2A mutation sgRNA

Plasmid

#186936

Purpose

sgRNA used for genetic mutation at Y81 and Y90 of Mcm2

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Mcm2-2A mutation sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole4 KO sgRNA

Plasmid

#186935

Purpose

Pole4 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole4 KO sgRNA (Pole4 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole3 KO sgRNA

Plasmid

#186934

Purpose

Pole3 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole3 KO sgRNA (Pole3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

Plasmid

#186418

Purpose

Expression of scrambled shRNA used as a control for knock down experiments

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

ScramblemiR

Use

AAV

Tags

mCitrine

Promoter

human Synapsin1

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Promoter

human Synapsin1

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst1-3xHA

Plasmid

#183540

Purpose

Express Dcst1-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP2-P2A-HO1-N1

Plasmid

#178966

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2-P2A-HO1

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP2-IRES-HO1-N1

Plasmid

#178967

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Tags

HO1 and no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1-H340D

Plasmid

#183362

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 H340D (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Mutation

H340D

Promoter

MESV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Mouse-caspase-1/11a

Plasmid

#183391

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Mouse, Synthetic)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Fus

Plasmid

#186145

Purpose

For expression of recombinant FAK using baculovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with FUS IDR

Promoter

polyhedrin promoter

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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FAK C-term

Plasmid

#186147

Purpose

For expresseion of recombinant FAK c-terminal domain in E. coli

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac FAK W266A

Plasmid

#186144

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

DNA mutation T796G;G797C Amino acid mutation W266A

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Lck

Plasmid

#186130

Purpose

For expression of recombinant Lck in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

Lck (Lck Mouse)

Tags

His6

Expression

Insect

Mutation

A1687T

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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