161,255 results

1. pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

   Plasmid

   #32703

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Cre/Lox and Retroviral

   Tags

   3x HA tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcmv3-IRG1-N152S-HA

   Plasmid

   #198182

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) N152S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The asparagine in IRG1 at position 152 mutates to…

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_smFP FLAG

   Plasmid

   #59756

   Purpose

   encodes multiple copies of an epitope tag FLAG

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-NFAT luciferase

   Plasmid

   #17870

   Purpose

   3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

   Depositor

   Jerry Crabtree

   Article

   Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

   Insert

   3x NFAT binding sequence (IL2 )
   

   Use

   Luciferase

   Tags

   IL2 promoter and Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   IL-2 promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYUBDuet

   Plasmid

   #37278

   Depositor

   Ted Baker

   Article

   Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

   Type

   Empty backbone

   Use

   Mycobacteria shuttle vector

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE-HA-NLS-I-PpoI

   Plasmid

   #46963

   Purpose

   For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   NLS-I-PpoI
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TET

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

   Plasmid

   #209283

   Purpose

   Expresses Voltron1 and jGCaMP7f in the zebrafish heart

   Depositor

   Adam Cohen

   Article

   Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

   Insert

   Voltron1_P2A_jGCaMP7f
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   zACNE20_myl7

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. hGli3 flag3x

   Plasmid

   #84921

   Purpose

   mammalian expression of Gli3

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli3 (GLI3 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB5318

    Plasmid

    #204828

    Purpose

    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetS promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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