176,159 results

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pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE2-TetOminiCMV-OSKM

Plasmid

#136541

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

Depositor

Hans Schöler

Publication

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBluescript-PB

Plasmid

#133885

Purpose

piggyBac ITR's in high copy number pBluescript backbone plasmid

Depositor

Mario Capecchi

Type

Empty backbone

Use

Piggybac

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

Plasmid

#136959

Purpose

Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

Depositor

Jakob Reiser

Publication

Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

Insert

Firefly luciferase and TurboRFP

Use

Lentiviral

Tags

V5 tag

Promoter

CMV

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-jRCaMP1b

Plasmid

#127873

Purpose

Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Publication

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Red-shifted mitochondrial matrix Ca2+ indicator

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-NLuc-IRES-Puro

Plasmid

#130936

Purpose

PiggyBac vector for constitutive NanoLuc expression in mammalian cells

Depositor

James Thomson

Publication

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NanoLuc

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-Rab7A

Plasmid

#131417

Purpose

Expresses HA-tagged human Rab7A in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

human Rab7A

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX TRE 3G BFP

Plasmid

#128070

Purpose

Lentiviral expression of Tet inducible TagBFP

Depositor

Oskar Laur

Insert

TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

no

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAR-Ec611

Plasmid

#130872

Purpose

A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

Depositor

Chang Liu

Publication

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Mutation

I777K, L900S

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAR-Ec318

Plasmid

#130871

Purpose

A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

Depositor

Chang Liu

Publication

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Expression

Mammalian

Promoter

UbC

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-H2B-iRFP720

Plasmid

#128961

Purpose

Lentiviral plasmid for expression of far red iRFP720 nuclear marker

Depositor

Carlos Carmona-Fontaine

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Promoter

hPGK

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

Plasmid

#106171

Purpose

cre mediated red to green shift

Depositor

Ewa Snaar-Jagalska

Publication

Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

Inserts

dsRED-express2
eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Publication

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Tags

His-Tag

Expression

Bacterial

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMaCTag-P07

Plasmid

#124788

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Publication

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Publication

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mIL12-N1

Plasmid

#123139

Purpose

Mammalian expression of murine cytokine IL-12 under CMV promoter

Depositor

Jordan Green

Publication

Tzeng et al Proc Natl Acad Sci U S A. 2020 Feb 7. pi

Insert

Murine IL12 tandem p40 p35

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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