We narrowed to 59,461 results for: %s

1. GFP-SpGalpha-i

   Plasmid

   #185568

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   Galpha-i
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   GFP, S-Tag

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYCTK006

   Plasmid

   #176710

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter SST2 (response part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   P-SST2
   

   Use

   Synthetic Biology

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYCTK007

   Plasmid

   #176711

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter YPS1 (response part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   P-YPS1
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYCTK016

   Plasmid

   #176720

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Saccharomyces cerevisiae (sender part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   mfalpha1Sc
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYCTK003

   Plasmid

   #176707

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS3 (response part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   P-FUS3
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH6HTC_PKM-Halo

   Plasmid

   #175321

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PKM (PKM Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDYU1B-TED

   Plasmid

   #154299

   Purpose

   A expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screening

   Depositor

   Shigehiko Yumura

   Article

   Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

   Type

   Empty backbone

   Use

   Dictyostelium expression

   Promoter

   actin15

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4133975

   Plasmid

   #152069

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;TapTag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGBW-m4134225

   Plasmid

   #152042

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. relAE335Q_NS1

    Plasmid

    #100917

    Purpose

    expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

    Insert

    RelA fragment from E.coli with point mutation E335Q
    

    Use

    Cyanobacteria cloning vector

    Tags

    FLAG

    Mutation

    E 335 converted to Q

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. rpaA_NS1

    Plasmid

    #101829

    Purpose

    drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA with its native promoter
    

    Use

    Cyanobacteria cloning vector

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJBAD87

    Plasmid

    #15353

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein(CSN)
    

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    F to S mutation

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

13. CRISPRoff-mScarletI

    Plasmid

    #217783

    Purpose

    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-pLKO-puro-SOX2

    Plasmid

    #47540

    Purpose

    Tet inducible knockdown of SOX2.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    SOX2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTripZ RB1 WT-3XFLAG

    Plasmid

    #209070

    Purpose

    DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag

    Depositor

    Matthew Oser

    Article

    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

    Insert

    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLD-puro-Cc-TARDBP-WT_VA

    Plasmid

    #141327

    Purpose

    Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-WT (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

    Expression

    Mammalian

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-SomaGCaMP7

    Plasmid

    #158759

    Purpose

    Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP7
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    synapsin promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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