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pBTK550d

Plasmid

#110617

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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MTK2_008

Plasmid

#123703

Purpose

Encodes human pUbc as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phUbC

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E836K)

Plasmid

#29542

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pOME0016

Plasmid

#178386

Purpose

Expresses KSHV ORF45 tagged

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF 45

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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BirA_BVES untagged

Plasmid

#196581

Purpose

expresses BirA

Depositor

Cheryl Arrowsmith

Article

Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

Insert

BirA_Ecoli

Use

Baculovirus expression

Available Since

June 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-KLF5-his

Plasmid

#40903

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD2_2

Plasmid

#36369

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E542K)

Plasmid

#29539

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pQTEV-GTF2F2

Plasmid

#31323

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

general transcription factor IIF (GTF2F2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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hGR-Dx

Plasmid

#133408

Purpose

overexpression of a mutant human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Mutation

N454D, A458T, R460D, D462C

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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PIGAZFN-L2

Plasmid

#37798

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger L2

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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L2-AA012

Plasmid

#185751

Purpose

Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Inserts

HygR2
eGFP

Tags

membrane localization tag Lti6b

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-Sirius

Plasmid

#175825

Purpose

Express Sirius with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD2_1

Plasmid

#36368

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
GST-FOXO3a 1-506aa

Plasmid

#10826

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

GST-FOXO3a 1-506aa (FOXO3 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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CTA2-mNG211

Plasmid

#141167

Purpose

Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

SUMO-CTA2-mNG211

Tags

SUMO

Expression

Bacterial

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S208A

Plasmid

#27115

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S208A

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
pminiT-Halo-MED1 donor

Plasmid

#197868

Purpose

Donor plasmid used for building Halo-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

Halotag

Promoter

NA

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

Plasmid

#91920

Purpose

Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcsCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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