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pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pEF1α-spider-monkey-retroCHMP3-HA

Plasmid

#154179

Purpose

expresses spider monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-B

Plasmid

#36996

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Plasmid

#125229

Purpose

Expresses transmembrane intracellular NanoLuc in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pLEX-MAPKAP1Del-FHH-IRES-Puro

Plasmid

#120706

Purpose

Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 Isoform 1

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Deleted amino acids 193-522

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pNB784

Plasmid

#60163

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 short UTR

Plasmid

#21648

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pJP72

Plasmid

#176479

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > GenR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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gRD21A-mRFP

Plasmid

#181959

Purpose

RD21A protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

RD21A genomic sequence including 2kb upstream of the ATG

Tags

mRFP

Expression

Plant

Promoter

RD21A native

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPN021

Plasmid

#91667

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC40

Plasmid

#62334

Purpose

sgRNA + 2x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Am

Plasmid

#109364

Purpose

Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Human, Branchiostoma belcheri)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pME18S-F-R-hb2AR

Plasmid

#149367

Purpose

Expresses human b2AR in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

b2AR (ADRB2 Human)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pIS1 Cyclin D2 long-mut-miR15/16

Plasmid

#21647

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 long-mut-miR15/16 (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR15/16 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only