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pTrichoGate-5

Plasmid

#160705

Purpose

Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_SARS-CoV_S

Plasmid

#207306

Purpose

Mammalian expression of Human SARS coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SARS-CoV_S (S Human SARS coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IRP1-Halo

Plasmid

#175305

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IRP1 (ACO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG5

Plasmid

#193807

Purpose

CMV overexpression of ChemoG5 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Ataxin3Q84

Plasmid

#22124

Depositor

Henry Paulson

Article

Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

Insert

Ataxin 3 (ATXN3 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-ATP

Plasmid

#193819

Purpose

CMV overexpression of ChemoB-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-ATP

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-0-SV40 polyA

Plasmid

#190864

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode0

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

Plasmid

#191146

Purpose

Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Tubulin-C-18

Plasmid

#57160

Purpose

Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Tubulin (TUBA1B Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

Plasmid

#163027

Purpose

FLiCRE TF with fast kinetics for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

CD4-MKII-f-hLOV1-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-2

Plasmid

#160702

Purpose

gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

gpdh

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-3-SV40 polyA

Plasmid

#190867

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode3

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-4-SV40 polyA

Plasmid

#190868

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode4

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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