We narrowed to 14,014 results for: TIM

1. pMX-hTAF11

   Plasmid

   #44341

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF11 (TAF11 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-LGC-53

   Plasmid

   #86399

   Purpose

   Mammalian expression of codon-optimized dopamine receptor LGC-53

   Depositor

   Steven Mennerick

   Article

   Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

   Insert

   LGC-53 (lgc-53 Nematode, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Codon optimized for mammalian expression; Signal …

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRAF6mut-UTR

   Plasmid

   #15094

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   TRAF6 3'UTR (TRAF6 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   4-bp substitutions in the miR-146 target sites

   Available Since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pTA231-p.Syn-PAmCherry1Hfx

   Plasmid

   #164661

   Purpose

   expression of H. volcanii codon-optimized PAmCherry1

   Depositor

   Anita Marchfelder

   Article

   Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

   Insert

   PAmCherry1Hfx
   

   Use

   Archaeal expression

   Mutation

   codon-optimization for Haloferax volcanii

   Promoter

   p.Syn

   Available Since

   Feb. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Keasling-2187

   Plasmid

   #100167

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   MevT and MBIS operon
   

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pclbw-PAGFPomp25

   Plasmid

   #58427

   Purpose

   Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   paGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   PAGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   cmv-bactin

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pTA231-p.Syn-Dendra2Hfx

   Plasmid

   #164660

   Purpose

   expression of H. volcanii codon-optimized Dendra2

   Depositor

   Anita Marchfelder

   Article

   Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

   Insert

   Dendra2Hfx
   

   Use

   Archaeal expression

   Mutation

   codon-optimization for Haloferax volcanii

   Promoter

   p.Syn

   Available Since

   Feb. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HeFm2SpCas9

   Plasmid

   #92111

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   “Highly enhanced Fidelity” mut2 SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. phumanCas9 (GB0575)

    Plasmid

    #68206

    Purpose

    Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-BE3-2X

    Plasmid

    #110835

    Purpose

    CMV expression vector for BE3-2X construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-2X
    

    Expression

    Mammalian

    Mutation

    2 tandem NLS sequences in the XTEN linker

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHcRed-miR-125b-1

    Plasmid

    #58989

    Purpose

    MSCV based expression vector - co-expresses HcRed and human miR-125b-1

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Inserts

    HcRed fluorescent protein
    miR-125b-1 (MIR125B1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Expression

    Mammalian

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RGB-S reporter

    Plasmid

    #207841

    Purpose

    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

    Depositor

    Christof Niemeyer

    Article

    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

    Inserts

    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    

    Expression

    Bacterial

    Promoter

    PosmY, PsulA, and grpE

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSII-PGK-FLPo-IRES-Puro

    Plasmid

    #205989

    Purpose

    Expresses Flp recombinase and puromycin resistance

    Depositor

    Sharon Torigoe

    Article

    Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

    Inserts

    PGK promoter
    FLPo (flp )
    IRES-PuroR cassette
    

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGM

    Plasmid

    #71250

    Purpose

    luciferase reporter with human GM-CSF promoter -627 to + 28

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF promoter -627 to +28 fragment (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge