We narrowed to 2,122 results for: Rp1;

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pAPM-miR30-ZC3HAV1-ts3

Plasmid

#174291

Purpose

ZC3HAV1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ZC3HAV1 shRNA (ZC3HAV1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1-L24_sfGFP

Plasmid

#207865

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1_sfGFP

Plasmid

#207801

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ1 ear

Plasmid

#199177

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ1 ear domain (Ap1g1 Mouse)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ2 ear

Plasmid

#199178

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ2 ear domain (AP1G2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2 Pab1 WT (123-204)

Plasmid

#196834

Purpose

Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

Pab1 (PAB1 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

123-204 amino acids

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2 Pab1 E181R (123-204)

Plasmid

#196835

Purpose

Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

Pab1 (PAB1 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

123-204 amino acids & E181R

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-tepsin

Plasmid

#196647

Purpose

Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Use

Yeast two-hybrid vector

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

silent substitution in codon 199 to eliminate int…

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pME-p3E-GLRX-roGFP2

Plasmid

#194288

Purpose

Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

Multisite gateway entry clone

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-NF-kB

Plasmid

#180206

Purpose

Expression GAL4-fusion of Co-NF-kB in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-1224

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCSN071

Plasmid

#166729

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-Mxi1-NLS

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCSN072

Plasmid

#166730

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1-NLS

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-BAF

Plasmid

#164248

Purpose

retroviral plasmid for GFP-BAF

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

BAF (BANF1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H

Plasmid

#163581

Purpose

express Dcp2C Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-300; mutate aa440-450, aa489-50…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAM1160c2

Plasmid

#159724

Purpose

Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPGIL-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1674

Plasmid

#159726

Purpose

Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPTLV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1479a3

Plasmid

#159725

Purpose

Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MYITV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-G96C/A97C/U100C/U101C

Plasmid

#82328

Purpose

U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C

Plasmid

#81238

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(G:H)

Plasmid

#138257

Purpose

Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

GFP targeting sgRNAs G and H

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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gh47

Plasmid

#106718

Purpose

expression of gRNA targeting ST6GALNAC6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GALNAC6 (ST6GALNAC6 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK504

Plasmid

#121459

Purpose

CEN HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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Hsh155 T178A R181A R182A R186A (N4A)

Plasmid

#111940

Purpose

This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutation

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 T178A R181A R182A R186A (N4A)

Expression

Bacterial and Yeast

Mutation

T178A R181A R182A R186A

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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