-
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG Barcode8
Plasmid#229070PurposeExpression mappingDepositorInsertCAG Barcode8
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG Barcode7
Plasmid#229069PurposeExpression mappingDepositorInsertCAG Barcode7
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pCAG-mock
Plasmid#228504PurposeThis plasmid can be used for control experiments and can also be used as a plasmid backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-protein-mock
Plasmid#228505PurposeThis plasmid can be used for control experiments and can also be used as a plasmid backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BiPOLES-mRuby3
Plasmid#227780PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3DepositorInsertBiPOLES-mRuby3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-TBK1
Plasmid#216709PurposeMammalian expression of TBK1DepositorInsertTBK1 (TBK1 Human)
UseTagsTSFExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)
Plasmid#173067PurposeExpresses NI-PE2DepositorInsertIgf1-NFATC2IP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
OgeuIscB CAG RNA
Plasmid#222867PurposeThis plasmid codes for the guide of the OgeuIscB with a CAG targetDepositorInsertOgeuIscB omega RNA with a (CAG)6 target sequence
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6Available sinceOct. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG_SRGAP2A_SH3(W765A)_GFP
Plasmid#222616PurposeExpresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.DepositorInsertSRGAP2 (SRGAP2 Human)
UseTagsEGFPExpressionMammalianMutationPoint mutation W765A (SH3 domain)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_SRGAP2A_SH3(W765A)_HA
Plasmid#222614PurposeExpresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.DepositorInsertSRGAP2 (SRGAP2 Human)
UseTagsHAExpressionMammalianMutationPoint mutation W765A (SH3 domain)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT46
Plasmid#218724PurposeExpresses N-terminally EGFP-tagged IFT46 in mammalian cellsDepositorInsertIFT46 (IFT46 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT172
Plasmid#218734PurposeExpresses N-terminally EGFP-tagged IFT172 in mammalian cellsDepositorInsertIFT172 (IFT172 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-HT(C199S)_WPRE
Plasmid#216415PurposeEncodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectorsDepositorInsertoROS-HT(C199S)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G_LF(C199S)
Plasmid#216116PurposeEncodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G_LF(C199S)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr_LF(C199S)
Plasmid#216114PurposeExpresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorInsertoROS-Gr_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CSII-CAG-EGFP
Plasmid#214112PurposeMammalian continuous expressionDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HaloTag-KRas4B(CT)
Plasmid#214276PurposeMammalian expression of HaloTag fused to the C-terminal tail of KRas4BDepositorInsertHaloTag7-KRas4B(174-188) (KRAS Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-NPHP1
Plasmid#209769PurposeHuman NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).DepositorInsertNPHP1 (NPHP1 Synthetic, Human)
UsePiggybac transposonTagsHA tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG101
Plasmid#210858PurposeMammalian expression of ATG101DepositorInsertATG101 (ATG101 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only