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K339CLM S330C
Plasmid#24441DepositorInsertKinesin (KIF5B Human)
UseTags6XHis and GFPExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S, S330CPromoterAvailable sinceMay 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
K343CLM A337C
Plasmid#24442DepositorInsertKinesin (KIF5B Human)
UseTags6XHis and GFPExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S, A337CPromoterAvailable sinceMay 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
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9E10.ChS78&81A.Gal4.pCDNA1
Plasmid#21901DepositorInsertCHOP (Ddit3 Mouse)
UseTagsGAL4-DNA-BD and MycExpressionMammalianMutationS78A S81APromoterAvailable sinceSept. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3
Plasmid#21795DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionMammalianMutationamino acids 1-50PromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 2: AIRAP(m1.9-T7)pGEX.KG
Plasmid#21792DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 L555A
Plasmid#19851DepositorInsertMKL1 L555A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 555 to AlaninePromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-GFP
Plasmid#126653PurposeInducible expression of Draper-GFP in S2 cellsDepositorInsertDraper
UseTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-delta ITAM-GFP
Plasmid#126655PurposeInducible expression of Draper-delta ITAM-GFP mutant in S2 cellsDepositorInsertDraper with ITAM Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic ITAM Tyr residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Crk-mCherry
Plasmid#126659PurposeInducible expression of Crk-mCherry in S2 cellsDepositorInsertCrk
UseTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT vCrk-GFP
Plasmid#126661PurposeInducible expression of vCrk truncation in S2 cellsDepositorInsertCrk
UseTagsExpressionInsectMutationresidues 1-182PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Ptp69D-GFP
Plasmid#126663PurposeInducible expression of Ptp69D-GFP in S2 cellsDepositorInsertPtp69D
UseTagsExpressionInsectMutationA53S, K105N, and A98T- please see depositor comme…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-GFP
Plasmid#126665PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain
UseTagsExpressionInsectMutationK986N- see depositor comments belowPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-mCherry
Plasmid#126666PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Shark-mCherry
Plasmid#126668PurposeInducible expression of Shark-mCherry in S2 cellsDepositorInsertShark mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper INT
Plasmid#126670PurposeExpression of His10-Draper intracellular domain for purification from E. coliDepositorInsertDraper cytoplasmic domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Lckg2a,Y394F)
Plasmid#177893PurposeBaculo expression of His10 tag fused to human LckG2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEVExpressionInsectMutationG2A,Y394FPromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
