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pAAV hSyn GFP-Fxr1

Plasmid

#112732

Purpose

AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

Depositor

Martin Beaulieu

Article

Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

Insert

Fxr1p (Fxr1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

hSYN

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pMVA2

Plasmid

#119951

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Expression

Bacterial

Mutation

A110G

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

Plasmid

#129667

Purpose

A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-U1AF37MF77M

Plasmid

#168267

Purpose

Expresses human dmU1A with the F37M/F77M double mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

U1A RRM1 crystallization module (SNRPA Human)

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F37M and F77M

Promoter

T7 promotor

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1472

Plasmid

#141825

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TOX2 (TOX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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3I91

Plasmid

#62514

Purpose

Bacterial expression plasmid for use in structural determination.

Depositor

Cheryl Arrowsmith

Article

Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

Insert

CBX8 (CBX8 Human)

Tags

6xHis and TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pBabe RFP-DN-FOXO1-HA neo

Plasmid

#45813

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA and RFP

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pTE3646

Plasmid

#137714

Purpose

Expresses LbCas12a RVRR variant in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

LbCas12a-RVRR

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.KAT5

Plasmid

#184652

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPc

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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DN1-Gli3 in AINGpCAGGS

Plasmid

#121970

Purpose

medium Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-864)

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727A-STAT3-3xFlag

Plasmid

#99262

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

S727A

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPn

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.DMAP1

Plasmid

#184650

Purpose

Retroviral expression of mouse DMAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only