We narrowed to 11,808 results for: KIN

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TTR C10S S85C A81T

Plasmid

#184745

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, A81T

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C K80E

Plasmid

#184744

Purpose

bacterial expression of huamn TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, K80E

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-FAK(397)FRB

Plasmid

#188660

Purpose

mVenus tagged Focal Adhesion Kinase with FRB inserted at Y397

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Focal Adhesion Kinase

Tags

mVenus

Expression

Mammalian

Mutation

Insertion of FRB at Tyrosine 397

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Mutation

K82R kinase-dead mutant

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1-H340D

Plasmid

#183362

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 H340D (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Mutation

H340D

Promoter

MESV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C I84S

Plasmid

#184748

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, I84S

Promoter

T7

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-H2BC11_sgRNA

Plasmid

#183883

Purpose

pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-MYH10_sgRNA

Plasmid

#183887

Purpose

pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLZRS-TrkC-mCherry

Plasmid

#182949

Purpose

To express the rat TrkC fused in the C-terminus with mCherry

Depositor

Pantelis Tsoulfas

Insert

TRk-C-mCherry (Ntrk3 Synthetic, Rat)

Use

Retroviral and Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

MMLV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-1-H340D

Plasmid

#183360

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 H340D (CASP1 Human)

Use

Retroviral

Tags

Myc

Mutation

H340D

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(33-520)

Plasmid

#183662

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 33-520

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pET Duet1 His6 SUMO D14

Plasmid

#177854

Purpose

Bacterial Expression of D14

Depositor

Karsten Melcher

Article

Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

Insert

D14

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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FG11F-C-FLAG-hNKD2

Plasmid

#174330

Purpose

encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

UbC

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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MO70-C-FLAG-hNKD2

Plasmid

#174327

Purpose

encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Retroviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 S573N

Plasmid

#167195

Purpose

puromycin resistant lentiviral vector for expression of PAK5 S573N

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 S573N

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_L139*

Plasmid

#174400

Purpose

S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

L139* (Truncation after 139th amino acid in sarA)

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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