179,359 results
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Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNme2ABE8e
TagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
ExpressionMammalianPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
Tagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPI-mEGFP
Plasmid#182866PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertGPI-mEGFP
ExpressionMammalianAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
TagsHis-tag followed by thrombin cleavage siteExpressionBacterialPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
TagsGSTExpressionBacterialPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU41
Plasmid#167663PurposeENTRY vector for gRNA1 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCShroom3 (Shroom3 Mouse)
UseGateway CloningTagsSspB and mCherryMutationIsoform X2, deleted aminoacids 1 - 1388Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
Plasmid#163476PurposeDirect-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpADepositorInsertiCre
UseAAVPromoterBeta Globin minimal promoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161926PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertrTetR(SE-G72P)
UseLentiviral and Synthetic BiologyTags3XFLAGExpressionMammalianPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OMMGFP 1-10
Plasmid#164119PurposeExpresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMMDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOMMGFP 1-10
ExpressionMammalianAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H2B-mGreenLantern
Plasmid#164464PurposeLocalization of mGreenLantern fluorescent protein to histone 2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGreenLantern
TagsHuman Histone 2B (D26G and V119I mutations)ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eMagAF-EGFP-PM
Plasmid#162247PurposeBlue-light dependent heterodimerization systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteMagAF-EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CTCF-mAC donor (Hygro)
Plasmid#140646PurposeCTCF tagging with mAID-cloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF (CTCF Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-hygro
Plasmid#160090PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX307-SARS-CoV-2-3CL
Plasmid#160278PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX301-ZIM3-KRAB-PYL1
Plasmid#154761PurposeExpresses ZIM3 KRAB domain fused to PYL1DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterCMVAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only