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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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wtBxb1

Plasmid

#222337

Purpose

Plasmid for expressing wild type Bxb1 in mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wild type Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-Benzonase

Plasmid

#218192

Purpose

Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

Depositor

Cheryl Arrowsmith

Article

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

MBP

Expression

Bacterial

Available Since

April 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHCMV_COCVmut_G

Plasmid

#207262

Purpose

Mammalian expression of a mutant Cocal virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

COCVmut_G

Expression

Mammalian

Mutation

K47A/Y209A/R354A; mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2_EF1a_TetOn_IRES_mCherry

Plasmid

#204155

Purpose

Lentivirus backbone expressing rtTA linked to mCherry

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

Tet-On 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-E01DARPin

Plasmid

#198287

Purpose

Produces PVCs retargeted with anti-EGFR DARPin E01

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-E01DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-Nme2ABE8e

Plasmid

#189928

Purpose

Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

Nme2ABE8e

Tags

NLS

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

Plasmid

#182287

Purpose

Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Inserts

codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT

Tags

nuclear export signal (NES)

Expression

Mammalian

Mutation

Y306A/Y384F (AF) double mutant of Methanosarcina …

Promoter

CMV and U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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HIF-TRE-mStrawberry reporter

Plasmid

#158681

Purpose

This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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RAR-TRE-mStrawberry reporter

Plasmid

#158683

Purpose

This plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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VPS13D^EGFP

Plasmid

#174109

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

EGFP

Expression

Mammalian

Mutation

With an internal EGFP tag in aminoacid position 1…

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-GST-His6-2_HBD

Plasmid

#171870

Purpose

Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Tandem repeats Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Article

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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