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169,371 results

Showing: 11101 - 11120 of 169371 results

  1. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  4. pBluescript-minimal PB

    Plasmid
    #177151
    Purpose
    Minimal piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177151-plasmid-map-sequence-id-348859

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  5. AAV-mDlx-jGCaMP8s-WPRE

    Plasmid
    #176755
    Purpose
    jGCaMP8s expression in GABA-ergic interneurons under the control of mDlx enhancer
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    mDlx
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176755-plasmid-map-sequence-id-349046

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  7. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Guoping Feng
    Has Service
    AAV PHP.eB and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192552-plasmid-map-sequence-id-403193

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  13. R777-E185 Hs.PLXNB1

    Plasmid
    #70469
    Purpose
    Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PLXNB1 (PLXNB1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70469-plasmid-map-sequence-id-125918

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  20. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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Showing: 11101 - 11120 of 169371 results