165,744 results

1. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCyCBE

   Plasmid

   #226505

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

   Depositor

   Weizhong Chen

   Article

   Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

   Inserts

   APOBEC1-Cas9D10A
   sgRNA
   sacB gene
   

   Use

   CRISPR

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCMV_CHIKV_E1E2

   Plasmid

   #207261

   Purpose

   Mammalian expression of Chikungunya virus E1E2 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-c2X

   Plasmid

   #75286

   Purpose

   Expresses proteins in the cytoplasm as fusions to maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 18, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJOE_pvdJ

   Plasmid

   #110142

   Purpose

   Suicide plasmid with repair template for knockout in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. P-rTetO-EGFP-T2A-RPL22-3xHA

   Plasmid

   #170326

   Purpose

   Tetracycline-inducible expression of HA and EGFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-Cas9

   Plasmid

   #196325

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding SpCas9 in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDC32-HPB

    Plasmid

    #32078

    Depositor

    Fumi Katagiri

    Article

    Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

    Type

    Empty backbone

    Use

    Gateway destination binary vector

    Tags

    HA-Prescission-Biotin

    Promoter

    35S promoter

    Available Since

    Dec. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-J chain

    Plasmid

    #145146

    Purpose

    Mammalian expression plasmid for J chain of polymeric antibody classes

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    immunoglobulin J chain (JCHAIN Human)
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-ZAP70

    Plasmid

    #23887

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-APOBEC1-YTHmut

    Plasmid

    #131637

    Purpose

    Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1-YTHmut-HA (YTHDF2 Human, Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Promoter

    CMV

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry KNL1 wt hardened

    Plasmid

    #45224

    Depositor

    Michael Lampson, Iain Cheeseman

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
    

    Tags

    TEV S15

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pLV-mTurquoise2-IL-1beta-mNeonGreen

    Plasmid

    #166783

    Purpose

    FRET analysis (pro-IL-1beta cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-IL-1b-mNeongreen (IL1B Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. mRNA-CleanCap-NanoLuc

    Plasmid

    #188247

    Purpose

    Template plasmid to transcribe mRNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. makeTCR_Lentiviral_Backbone

    Plasmid

    #233943

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a (long)

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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