We narrowed to 14,255 results for: TIM
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Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-guide1-PGK-Puro
Plasmid#102904PurposeEBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFPāCOSMOāPB
Plasmid#169684PurposeCOSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1āPB tagExpressionMammalianMutationY104WPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Hygro(-)-B2-cT
Plasmid#104355PurposeExpresses human beta 2 integrin tension sensorDepositorAvailable SinceDec. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaLgp160-754*
Plasmid#123212PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (BaL) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop coā¦PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR2956
Plasmid#122533PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2
ExpressionBacterialPromoterPnhx-2Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (Merlin)
Plasmid#136448PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Bongwoori
Plasmid#63720PurposeConsists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227DDepositorInsertBongwoori
TagsSuper ecliptic pHlorin A227DExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoter35SAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gi-c009
Plasmid#173079PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)
Plasmid#55199PurposeCFP reporter along with FF4 miRNA binding sites and 28 nt sequencesDepositorInsertECFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNa12_opt.IRES.eGFP
Plasmid#22490DepositorAvailable SinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOP459
Plasmid#85933PurposeEncodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.DepositorInsertAnti-Ebola 2G4 monoclonal antibody
ExpressionYeastPromoterAOX1Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only