164,826 results

1. pCIG3 (pCMV-IRES-GFP version 3)

   Plasmid

   #78264

   Purpose

   Lentiviral empty cloning vector containing IRES and GFP

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PE-Max-pet21A

   Plasmid

   #204471

   Purpose

   Expression plasmid for PE protein in bacteria.

   Depositor

   Scot Wolfe

   Article

   Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

   Insert

   Prime editor
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pELPS-eDHFR-YFP-T2A-Renilla

   Plasmid

   #193253

   Purpose

   Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

   Depositor

   Mark Sellmyer

   Article

   Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

   Insert

   The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Renilla luciferase (rLuc) and yellow fluorescent …

   Expression

   Mutation

   None

   Promoter

   EF1a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

   Plasmid

   #128174

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMPRA3-CMV-Clover

   Plasmid

   #53540

   Purpose

   Fluorescent reporter control for pMPRA3

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   Clover GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBHt2G-RFP

   Plasmid

   #107162

   Purpose

   Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

   Depositor

   Sophien Kamoun

   Type

   Empty backbone

   Use

   Fungal grobacterium transformation plasmid, teste…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. RAB14 shRNA

   Plasmid

   #61496

   Purpose

   to knockdown endogenous RAB14 expression levels

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   RAB14 (RAB14 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-NPM1c-IRES-mCherry

   Plasmid

   #131825

   Purpose

   Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. hGli2 flag3x

    Plasmid

    #84920

    Purpose

    mammalian expression of Gli2

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli2 (GLI2 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIB1-CreC(N1)

    Plasmid

    #75367

    Purpose

    Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Cre DNA recombinase, residues 106-343

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-miniGFP2-P2A-FusionRed

    Plasmid

    #188734

    Purpose

    Express miniGFP2 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-NTOM20-mVenus-Zdk1

    Plasmid

    #81010

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJExpress414_GmPOPB-S577A

    Plasmid

    #92235

    Purpose

    Express S577A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-S577A mutant
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Serine 577 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLpA-DN.JNK1

    Plasmid

    #51942

    Purpose

    Adenoviral vector for dominant negative JNK1 expression

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    DN JNK1 (Mapk8 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mutation

    dominant negative: the dual phosphorylation moti…

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pDule2-Acd82

    Plasmid

    #174100

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG_iPE-C

    Plasmid

    #214735

    Purpose

    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-C_bGH
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCP60 RS-GFP

    Plasmid

    #122173

    Purpose

    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

    Depositor

    Lukas Fischer

    Article

    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

    Insert

    RS-GFP
    

    Use

    Binary vector for escherichia coli and agrobacter…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIVT-eeBxb1

    Plasmid

    #232748

    Purpose

    IVT template for making modRNA encoding eeBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    V74A, E229K, V375I

    Promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-li-p33_RS-CLIP

    Plasmid

    #104634

    Purpose

    Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

    Depositor

    Sébastien Walchli

    Article

    Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

    Insert

    modified Invariant chain p33 (CD74 Human)
    

    Use

    Entry vector for gateway system

    Tags

    none

    Expression

    Mutation

    CLIP domain was replaced by 2 restriction sites

    Promoter

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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