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We narrowed to 11,509 results for: ARIA;

Showing: 11141 - 11160 of 11509 results
  1. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

    Plasmid
    #212504
    Purpose
    Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production
    Depositor
    Insert
    SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    See Depositor Comments.
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

    Plasmid
    #217802
    Purpose
    Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. PCDF Bravo-PotH-OL3/2sub

    Plasmid
    #216752
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Insert
    potH (potH E. coli (Bacteria))
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCMV6-AN-DDK_WT RAD23A_delta aa 230-285 (delta XPC-binding domain)

    Plasmid
    #201464
    Purpose
    Expresses a mutant form of human RAD23A that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
    Depositor
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 230-285
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. hTln1-R1R2-17b

    Plasmid
    #191427
    Purpose
    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria
    Depositor
    Insert
    Human Talin1 R1R2 domains (TLN1 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    contains 17AA insertion from inclusion of exon 17b
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid
    #129793
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G315T
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid
    #129789
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    W99A
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid
    #129790
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    N51L
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 11141 - 11160 of 11509 results