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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

Plasmid

#174133

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

Plasmid

#174135

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEZT-GST-SLFN2-T2

Plasmid

#174321

Purpose

Expression of recombinant GST-SLFN2-K242A, K249A, Q301A

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GST-SLFN2-K242A, K249A, Q301A (Slfn2 Mouse)

Use

Bacmam, baculovirus

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMbd3EosHaloTAF_Hyg

Plasmid

#140958

Purpose

Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

methyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMta2-EosHalo-TAFiP

Plasmid

#140959

Purpose

Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pChd4EosHaloTAF_Puro

Plasmid

#172099

Purpose

Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Noxa)-pEGFP-C1

Plasmid

#166761

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Noxa BH3) (Bcl2l11, PMAIP1 Mouse, Human)

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Noxa…

Promoter

CMV

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)

Plasmid

#174712

Purpose

Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Tags

3xEGFP

Expression

Mammalian

Mutation

mMeikin: 1-391, E156R, R159E; hRad21: 142-275; mM…

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Kctd19-3xHA

Plasmid

#173497

Purpose

Express Kctd19-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

Insert

Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434)

Plasmid

#174713

Purpose

Mammalian expression of mouse Meikin with inserted C-terminal Separase cleavage site inactivated

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Tags

3xEGFP

Expression

Mammalian

Mutation

mMeikin: 1-391; hRad21: 142-275, E169R, R172E; mM…

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pME-MmCavin4

Plasmid

#170528

Purpose

Entry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADM

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-APEX2

Plasmid

#170526

Purpose

Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Leishmania cell free expression clone

Tags

APEX2 and Lifeact

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_3xFLAG-Kctd19

Plasmid

#173498

Purpose

Express 3xFLAG-Kctd19 under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

Insert

Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pME-MmCav1

Plasmid

#170527

Purpose

Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Multisite gateway entry clone

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

Plasmid

#135563

Purpose

An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (scrambled)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-mouse_min2

Plasmid

#173965

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#3

Plasmid

#169791

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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