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Plasmid#47796PurposeExpresses enzymatically monobiotinylated full-length RAP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorPublicationInsertCodon-optimised RAP3
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX62
Plasmid#46854PurposeTOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCEDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertattB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
ExpressionMammalianMutationG245SAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTK158_mCherry-4.1G-deltaCTD-hardened
Plasmid#46360DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4.1G (EPB41L2 Human)
TagsmCherryExpressionMammalianMutationDeleted amino acids 886-1005, and modified for si…PromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUASP YFP Rab5 DN
Plasmid#37694DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS43N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1DCH2AB
Plasmid#13472DepositorInsertMKP-1DCH2AB (Dusp1 Mouse)
TagsEGFPExpressionMammalianMutationDeletion of MKP-1 CH2AB; contains aa 137-367Available sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro
Plasmid#228401PurposeEncodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSREBP1 (SREBF1 Human)
TagsmNeonGreen2xExpressionMammalianMutationdeleted N terminal activation domain (amino acids…PromoterEF1aAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2
Plasmid#202762PurposeEncodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harborDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNEUROG2 (NEUROG2 Human)
ExpressionMammalianPromoterTetOAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a-KRAB_crRNA NC
Plasmid#176262PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleasDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseMutationD917A and E1006A mutations to inactivate the endo…Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y3F-PolyA
Plasmid#112288PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutation3 Tyrosines (341, 357 and 394 corresponding to ty…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
ExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
vsv-CenpB 1-166-Sgo1-GFP
Plasmid#108495Purposeexpression of vsv-CenpB-1-166-Sgo1-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCenpB-Shugoshin 1 (SGO1 Human)
TagsEGFP and VSVExpressionMammalianMutationCenpB 1-166 fusion before Sgo1PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_Arg322Lys
Plasmid#105849PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
ExpressionMammalianMutationArg322Lys in constant region of IgG1 heavy chainPromoterhEF1-HTLVAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(150-453)
Plasmid#90109PurposeMammalian expression of Flag-MCPIP1(150-453)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCPIP1 (ZC3H12A Human)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRCA1 (BRCA1 Human)
Tags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSALECTNK-TEM1(S70A,D179G)/csTEM1
Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
TagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SLAP-FLAG
Plasmid#74500PurposeOverexpression of SLAP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSLAP (SLA Human)
ExpressionMammalianMutationN18K, G74A, and T149APromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS Exon Vector
Plasmid#69884PurposeExpression plasmid for expressing circular RNAs of a desired sequence in DrosophilaDepositorPublicationAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
P92-bio
Plasmid#68520PurposeExpresses full-length P. vivax P92 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP92
Tagsrat CD4 d3+4, enzymatic biotinylation sequenceExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP4-bio
Plasmid#68510PurposeExpresses full-length P. vivax MSP4 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMSP4
Tagsrat CD4 d3+4, enzymatic biotinylation sequenceExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only