We narrowed to 22,307 results for: CRISPR

1. pMpGE011

   Plasmid

   #71537

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAtC

   Plasmid

   #78097

   Purpose

   Plant expression of Cas9 and gRNA empty backbone; Basta resistance

   Depositor

   Jin-Soo Kim

   Article

   Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NLS-HA

   Expression

   Plant

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFnCas12a_NT

   Plasmid

   #169838

   Purpose

   Expresses FnCas12a and guide RNA in Bacteria

   Depositor

   John van der Oost

   Article

   Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXynA

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAtC

   Plasmid

   #78098

   Purpose

   Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

   Depositor

   Jin-Soo Kim, Sang-Gyu Kim

   Article

   Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV1326

   Plasmid

   #111435

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC10

   Plasmid

   #104784

   Purpose

   pAH595-gRNA 2xplex entry cassette

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNHEJ-Cas12a-recX

   Plasmid

   #158714

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pmyc

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNHEJ-Cas9-recX

   Plasmid

   #158715

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDuRCC-N

   Plasmid

   #81194

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please view depositor comments below and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS433-pCSDest2-AcrIIC1Nme

    Plasmid

    #85679

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC1Nme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPbuCas13b-gRNA-NT

    Plasmid

    #184568

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLsCas13aGG

    Plasmid

    #131012

    Purpose

    Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of LsCas13a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS436-pCSDest2-AcrIIC2Nme

    Plasmid

    #85712

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC2Nme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS443-pCSDest2-AcrIIC3Nme

    Plasmid

    #85713

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC3Nme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS430-pCSDest2-AcrIIC1Boe

    Plasmid

    #85678

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC1Boe
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPgiCas13b-gRNA-NT

    Plasmid

    #184835

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS424-ysg(G:H)

    Plasmid

    #138257

    Purpose

    Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    GFP targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS427-pCSDest2-AcrE2

    Plasmid

    #85677

    Purpose

    Mammalian expression of anti-CRISPR protein from Type I-E

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type I-E anti-CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. XClone-Cas9-Cronus-T2A-BFP

    Plasmid

    #141209

    Purpose

    Tunable and temporal expression control of Cas9

    Depositor

    Rory Johnson

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. dCas9-3×RFP-P2A-CASANOVA/gRNA telo

    Plasmid

    #137711

    Purpose

    Vector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA telo

    Depositor

    Dominik Niopek

    Insert

    dCas9-3×RFP-P2A-CASANOVA/gRNA telo
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo

    Plasmid

    #137712

    Purpose

    Vector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo

    Depositor

    Dominik Niopek

    Insert

    dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJKW0474

    Plasmid

    #107588

    Purpose

    pCLV3:Cas9

    Depositor

    Jing-Ke Weng

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis CLAVATA3

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pJKW0457

    Plasmid

    #107586

    Purpose

    AtU6-26 sgRNA

    Depositor

    Jing-Ke Weng

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pENTR_AtU6p_BsaI_gRNA

    Plasmid

    #225987

    Purpose

    To make Gateway entry clone of CRISPR guide RNA under AtU6p

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid

    #196712

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-KRAB(ZIM3)-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX552-GFP

    Plasmid

    #107023

    Purpose

    pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.

    Depositor

    Alex Hewitt

    Insert

    GFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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