We narrowed to 7,692 results for: Lif;
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Plasmid#160054PurposeMammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1
Plasmid#197421PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
UseCRISPRAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2
Plasmid#197422PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
UseCRISPRAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3
Plasmid#197423PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
UseCRISPRAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1
Plasmid#197424Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
ExpressionMammalianAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2
Plasmid#197425Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
ExpressionMammalianAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mCsnk2b - 2
Plasmid#198506Purposelentiviral stable expression of mCsnk2b gRNA 2DepositorAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA resistant ABHD16A-S176AS306A-mNG
Plasmid#195165Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-mNG
Plasmid#195166Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-mNG
Plasmid#195167Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-Halo
Plasmid#195168Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
TagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-Halo
Plasmid#195169Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
TagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.15_pT-REX17
Plasmid#195497PurposeFirefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17DepositorInsertPromoter region of lncRNA T-REX17
Tagsluc2-hPESTExpressionMammalianAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E,K450R)
Plasmid#187881PurposeGal4/UAS expression of dSarm(isoE,K450R)DepositorInsertdSarm isoform E (Sarm )
MutationK450RAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP
Plasmid#190039PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
TagsGST and MBPExpressionMammalianMutation(641-779)deletion & 1363 RDKDLIES 1370 to GSS…PromoterCMVAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα
Plasmid#183454PurposesgRNA targeting rat PKA-RIIα subunitsDepositorInsertsgRNA targeting rat PKA-RIIα (Prkar2a Rat)
UseLentiviralPromotershRNA: H1 / gene: ubiquitinAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8322 pDONR ATG-YAP1 S94A-Stop
Plasmid#184526PurposeSubcloning YAP1 S94ADepositorAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8321 pDONR ATG-YAP1 S127A-Stop
Plasmid#184525PurposeSubcloning YAP1 S127ADepositorAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-50-DExogron-mCherry-R11b
Plasmid#179904PurposeDonor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-23-DExCon-mCherry-R11b
Plasmid#179898PurposeDonor with homologous arms for Rab11b to knock in DExCon-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-67-DExCon-mNeonGreen-R11b
Plasmid#179899PurposeDonor with homologous arms for Rab11b to knock in DExCon-mNeonGreen moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-59-DExCon-mTagBFP2-R25
Plasmid#179900PurposeDonor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmTagBFP2ExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsmEGFPMutationreplaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…PromoterSFFVAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-anti-MIR328-3p
Plasmid#153319PurposeExpresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.DepositorInsertzsGreen with MIR328-3p sponge
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-anti-MIR211-5p
Plasmid#153318PurposeExpresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.DepositorInsertzsGreen with MIR211-5p sponge
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo
Plasmid#179440PurposeExpresses rTetR-HDAC4(human) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-HDAC4
ExpressionMammalianPromoterpGKAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
iDianiSnFR
Plasmid#177741PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiDianiSnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2
Plasmid#175751PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A
Plasmid#175752PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50ADepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CsoS2 ΔNTD
Plasmid#162705PurposeCarboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2DepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationDeletion of residues 2-256 of CsoS2, Y131DPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B H85E K88E I92E
Plasmid#175033PurposeExpresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (H85E K88E I92E) (WIPI2 Human)
TagsHaloTagExpressionMammalianMutationHistadine 85 to Glutamate (CAC to GAG); Lysine 88…PromoterCMVAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCytSnFR
Plasmid#177738PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCytSnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_BrEt_SnFR
Plasmid#177740PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_BrEt_SnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_F_SnFR
Plasmid#177739PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_F_SnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ C20A
Plasmid#172112PurposeExpression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequenceDepositorInsertMethionine Binding Protein
Tags10x Histidine Tag and N.meningitidis signal seque…ExpressionBacterialMutationChanged Proline 22 to Alanine on MetQ and Cystein…PromoterT7 PromoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA2
Plasmid#171000PurposeHiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A, R538A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[R537A, R538A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA5
Plasmid#171003PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/R
Plasmid#166976PurposeExpresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AQ/Y
Plasmid#166975PurposeExpresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3486 (YCp LEU2 Rpb1 F1492A)
Plasmid#91808PurposeYeast expression of S. cerevisiae Rpb1 with a F1492A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged phenylalanine 1492 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3487 (YCp LEU2 Rpb1 S1493A)
Plasmid#91809PurposeYeast expression of S. cerevisiae Rpb1 with a S1493A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged serine 1493 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3488 (YCp LEU2 Rpb1 P1494A)
Plasmid#91810PurposeYeast expression of S. cerevisiae Rpb1 with a P1494A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged proline 1494 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3508 (YCp LEU2 Rpb1 Y1473A)
Plasmid#91811PurposeYeast expression of S. cerevisiae Rpb1 with a Y1473A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged tyrosine 1473 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3509 (YCp LEU2 Rpb1 T1471A)
Plasmid#91812PurposeYeast expression of S. cerevisiae Rpb1 with a T1471A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged threonine 1471 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3511 (YCp LEU2 Rpb1 P1472A)
Plasmid#91813PurposeYeast expression of S. cerevisiae Rpb1 with a P1472A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged proline 1472 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3567 (pET151_Spt6 1247-1451 R1282H)
Plasmid#91816PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged arginine 1282 to histidine; deleted resid…PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3495 (pET151_Spt6 1247-1451 K1435A)
Plasmid#91817PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged lysine 1435 to alanine; deleted residues …PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ CbbQ-
Plasmid#162711PurposeSecond CCM operon with inactive CbbQ (K46A, E107Q) rubisco activaseDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationInactivation of CbbQ subunit of CbbOQ rubisco act…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ ΔacRAF
Plasmid#162710PurposeSecond CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAFDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationpCCM' with a deletion of putative rubisco ch…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only