We narrowed to 2,961 results for: NAK

1. pMXs-hs-3xHA-delta6xNHL-LIN-41

   Plasmid

   #52722

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 593–868

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pONSY-CoNMM:mCherry

   Plasmid

   #111878

   Purpose

   This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-hs-3xHA-deltaRING-LIN-41

   Plasmid

   #52718

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 12-91

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. dmBACCS2VL-IRES-dOrai-IRES-mCherry

   Plasmid

   #72898

   Purpose

   Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2VL-IRES-dOrai-IRES-mCherry
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

   Plasmid

   #173866

   Purpose

   Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. dmBACCS2NS-IRES-dOrai-IRES-mCherry

   Plasmid

   #72896

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai-IRES-mCherry
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pONSY-CoH2B:Venus

   Plasmid

   #111877

   Purpose

   This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Capsaspora Histone H2B (CoH2B) fused to Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

   Plasmid

   #173861

   Purpose

   Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

   Plasmid

   #159894

   Purpose

   AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

   Insert

   NES-tdTomato-LINuS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α promoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid

    #173865

    Purpose

    Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dmBACCS2VL-IRES-dOrai-IRES-GFP

    Plasmid

    #72897

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

    Plasmid

    #52720

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 328–447

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-hs-3xHA-C12AC15A-LIN-41

    Plasmid

    #52725

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed cysteines 12 and 15 to alanines

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    changed 478 Tyrosine for Alanine

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-hs-3xHA-deltaBBox-LIN-41

    Plasmid

    #52719

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 194-320

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-hs-3xHA-deltaFilamin-LIN-41

    Plasmid

    #52721

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the Filamin domain and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 483–583

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4c/His-Xpress-HA-hBIG2(WT)

    Plasmid

    #79432

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4c/His-Xpress-HA-hBIG1(WT)

    Plasmid

    #79431

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    C1758R

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA.5.1-FHS-TNRC6A-SD-S1616A/S1691A

    Plasmid

    #215909

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1616A, S1691A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D

    Plasmid

    #215912

    Purpose

    Expression of full-length TNRC6A with S1332D/S1346D mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    Full length of TNRC6A, S1332D, S1346D

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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