We narrowed to 9,742 results for: Tec;
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Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-GalT
Plasmid#11930DepositorInsertGalT (GALT Human)
ExpressionMammalianMutationAmino acids 1-60 of the human galactosyltransfera…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-MKLP1
Plasmid#70154Purposemammalian expression of MKLP1 with an mCherry fusionDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2
Plasmid#69623PurposeA FRET sensor for Galphai1 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2
Tagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianPromoterCMV and CMV (IRES)Available SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
TagsMet-6xHisExpressionBacterialPromoterT5 promoterAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX neo PFKP-GFP
Plasmid#116940PurposeExpression of PFKP-GFP fusion proteinDepositorAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2
Plasmid#69624PurposeA FRET sensor for Galphai2 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2
Tagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianPromoterCMV and CMV (IRES)Available SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2
Plasmid#69625PurposeA FRET sensor for Galphai3 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2
Tagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianPromoterCMV and CMV (IRES)Available SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ECFPEYFP
Plasmid#24520DepositorInsertECFP, EYFP
TagsECFP and EYFPExpressionMammalianAvailable SinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDDRFP-A1
Plasmid#36289DepositorInsertDimerization dependent RFP A1
TagsHis tagExpressionBacterialAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDDRFP-B1
Plasmid#36290DepositorInsertDimerization dependent RFP-B1
TagsHisExpressionBacterialAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CreEGFP
Plasmid#165140PurposeExpression of Cre recombinase fused with EGFP in C-terminalDepositorInsertCre recombinase
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP
Plasmid#52675PurposeshRNA silencing plasmid with eGFP transfection markerDepositorTypeEmpty backboneUseRNAiTagsCMV-EGFPPromoterCMV and U6Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Rab7
Plasmid#129603PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorInsertRab7
ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only