We narrowed to 11,800 results for: emb
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Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralExpressionMammalianAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EasyFusion T2A-H2B-mCherry
Plasmid#113089PurposeGeneral donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT2A-H2B-mCherry
UseSynthetic BiologyAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRExpressionMammalianPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330a dCas9-VP64
Plasmid#92363PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-VP64
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-CB-FLPe-P2A-tdTomato
Plasmid#72259PurposeExpress FLPe and tdTomato under CB promoterDepositorInsertFLPe-P2A-tdTomato
UseLentiviralExpressionMammalianMutationT230I in tdTomato (please see depositor's co…PromoterCB promoter and chicken beta-actin + CMV enhancerAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:Renilla:Tnos (GB0109)
Plasmid#68215PurposeTranscriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoterDepositorInsertRenilla
UseLuciferase and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BirA His-tag pLEMO
Plasmid#119817PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
Tags6-Histidine-tagExpressionBacterialAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXN21-Dest
Plasmid#105086PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-BioID-FAM21
Plasmid#121046PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsGFPExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP Tubulin K40R
Plasmid#105303PurposeTo study effects of acetylation-null tubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha tubulin (TUBA1B Human)
TagsEGFPExpressionMammalianMutationK40RAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
nAChR alpha4 YFP
Plasmid#15245DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha4-YFP-M (Chrna4 Mouse)
TagsYFPExpressionMammalianAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthAlpha7 (CHRNA7 Human)
ExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-P4Mx1
Plasmid#108143PurposePI4P biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertX.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
ExpressionMammalianPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-EF1s
Plasmid#72484PurposeExpress gene of interest under truncated EF-1 promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJan. 15, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
ER-RA
Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_3xFLAG-ATP6V0A3-mScarlet
Plasmid#228867PurposeStable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarletDepositorAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
726-Desmoglein2-myc
Plasmid#32232DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDesmoglein2 (DSG2 Human)
TagsMycExpressionMammalianPromoterhuman beta-actinAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-CMV-TetO2_3C-Avi-His6
Plasmid#113887PurposeMammalian (inducible) protein expression, in vivo biotinylationDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6PromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mito-GA
Plasmid#209865PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP A
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only