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pJUMP24-1A(sfGFP)

Plasmid

#126971

Purpose

Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJC7

Plasmid

#177872

Purpose

Co-express in mammalian cells two target proteins, using an internal ribosome entry site and two multi-cloning sites.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Axud1_enh_394_Citrine

Plasmid

#130625

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Axud1_enh_394

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D3

Plasmid

#10912

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D3 (CCND3 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSBDEST.IP

Plasmid

#79472

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pPBpuro-hM3D-P2Av3-mCherryNLS

Plasmid

#184714

Purpose

Gq DREADD

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

hM3D

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-HIS-FLAG-AtUBQ11

Plasmid

#222720

Purpose

Expression HIS-FLAG-AtUBQ11 in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBQ11 (UBQ11 Mustard Weed)

Tags

2xFLAG and 6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-RAB3A-WT

Plasmid

#206146

Purpose

PGK EGFP-RAB3A-WT

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

RAB3A (RAB3A Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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APPL1-pmCherryC1

Plasmid

#27683

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

APPL1 (Appl1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

Plasmid

#58433

Purpose

Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)2-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Promoter

UAS-hsp70

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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PKC beta II CAT

Plasmid

#16384

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-673

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+ cas

Plasmid

#26729

Depositor

Didier Stainier

Article

Kikuchi et al Genes Dev. 2001 Jun 15. 15(12):1493-505.

Insert

casanova (sox32 Zebrafish)

Use

Xenopus/mammalian/avian/zebrafish

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-13

Plasmid

#119992

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-sfGFP

Plasmid

#189675

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-0-T7-GFP_ColE1

Plasmid

#171649

Purpose

QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-0-T7

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-10-T7-GFP_ColE1

Plasmid

#171654

Purpose

QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-10-T7

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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