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pAAV-CMVenh synapsin-intron-eGFP

Plasmid

#247941

Purpose

AAV transfer plasmid encoding enhanced Green Fluoresence Protein control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Oliveras-Salva et al Mol Neurodegener. 2013 Nov 25;8:44. doi:

Insert

eGFP

Use

AAV

Promoter

CMVenh synapsin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBMN mEGFP-OPTN

Plasmid

#188784

Purpose

For mammalian expression of OPTN tagged with mEGFP

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_sfGFP3TCG4TAG

Plasmid

#174518

Purpose

Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

sfGFP

Tags

6 x His

Expression

Bacterial

Mutation

Recoded. TCG and TAG codons were introduced in po…

Promoter

araBAD

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein

Plasmid

#117842

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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HSC1-HS4-D4Z4c-GiP

Plasmid

#58541

Purpose

Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-iLIDx6

Plasmid

#103779

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

mCherry-iLIDx6

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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HA-hNM2B

Plasmid

#236533

Purpose

For expression of NM2B (human) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH10 (MYH10 Human)

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-N-Myc

Plasmid

#50772

Purpose

retroviral expression of human N-Myc

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

N-myc (MYCN Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pBMN mEGFP-NDP52

Plasmid

#188785

Purpose

For mammalian expression of NDP52 tagged with mEGFP

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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cyto-CFP-FRBx5

Plasmid

#103776

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-CFP-FRBx5

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG PIK3CA Wildtype

Plasmid

#73056

Purpose

Human PIK3CA Wildtype

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA Wildtype (PIK3CA Human)

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG

Plasmid

#248670

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo full length VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL (VHL Human)

Tags

FLAG-miniTurbo and NLS

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ166-OsPE2

Plasmid

#141080

Purpose

Gateway entry clone for CRISPR-Cas9(H840A) prime editing

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Insert

zCas9(H840A)-OsM-MLV-RT

Use

CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

Tags

3x FLAG, NLS and NLS

Expression

Plant

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN HA-NDP52

Plasmid

#199307

Purpose

For expression of the mammalian NDP52 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human CIN85

Plasmid

#47935

Purpose

expresses Myc tagged human CIN85 in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

CIN85 (SH3KBP1 Human)

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 2-665 of human CIN85; D114G

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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